刺五加皂苷对外周刺激诱发的海马长时程增强效应的影响

目的：探讨刺五加皂苷（ASS）对大鼠海马CA1区长时程增强效应（LTP）的影响。方法：20只雄性SD大鼠随机分为对照组与ASS组,每组10只,两组单刺激（10～20 V,0.5 ms,1次/min,30 min）坐骨神经诱发海马CA1区场电位。强直刺激（35 V,0.5 ms,100 Hz,持续1 s,间隔10 s,4次）坐骨神经,诱发海马CA1区场电位LTP。ASS组在强直刺激前,于侧脑室内微量注入ASS 1.0μg/5μL,强直刺激后观察其对LTP的影响。结果：电刺激坐骨神经可在海马CA1区诱发出潜伏期（172.33±1.65）ms和可重复出现的以负波为主的场电位,平均幅度（32.24±0.72）μV。对照组强直刺激后可出现持续时间2 h以上的LTP现象。ASS增强了强直刺激后海马CA1区LTP的诱导。结论：刺五加皂苷对大鼠海马CA1区LTP有明显促进作用,表明其可提高大脑学习、记忆能力。

Effect of Acanthopannx Senticousus Saponins on Hippocampal CA1 LTP induced by Peripheral Stimulation

Objective ：To investigate the effect of acanthopannx senticousus saponins （ASS）on long-term potentiation （LTP）in hippocampal CA1 induced by peripheral stimulation. Methods：20 male SD rats were randomized into control group and ASS group, 10 rats in each one. Two trains of single twitch stimulation （10V-20 V,0.5 ms, 1/min for 30 min） were applied to sciatic nerve to induce field potentials of hippocampal CA1. Tetanic stimulation （100 Hz,35 V,0.5 ms, lasting 1 s,at the interval of 10 s,4 times）was delivered to induce LTP in hippocampal CA1. In ASS group,before tetanic stimulation, ASS 1.0 μg/5μl was injected into intracerebroventricule （i.c.v）, and afterwards,the effect of ASS on LTP was observed. Results：Electric stimulation in sciatic nerve induced latency（172.33±1.65ms）and field potentials that were preented repeatedly with negative wave predominated,and of mean amplitude（32.24±0.72 μV）in hippocampal CA1. In control group,after tetanic stimulation,LTP was continued for over 2 h. ASS enhanced LTP induction in hippocampal CA1 after tetanic stimulation. Conclusion：Acanthopannx senticousus saponins promotes remarkably LTP in hippocampal CA1 in rats.which indicates that it improves cerebral learning and memory.