常规检测法与革兰染色法在阴道分泌物检测中的应用价值探讨

目的 探讨常规检测法(湿片镜检法和五联检测法)与革兰染色法在阴道分泌物检测中的应用价值。方法 选取2021年1月至2021年12月浙江大学医学院附属金华医院就诊的479例女性阴道炎患者作为研究对象,分别采用常规检测法与革兰染色法对阴道分泌物标本进行检测,对检测结果进行比较分析。结果 常规检测法和革兰染色法的Kappa值为0.793,具有较高的一致性(P<0.05)。在白细胞的检出率上,常规检测法高于革兰染色法,差异有统计学意义(P<0.05)。在致病性细菌、乳酸杆菌的检出率上,革兰染色法远高于常规检测法,差异有统计学意义(P<0.05)。结论 在日常工作中,常规检测法能较好的完成日常阴道分泌物筛查工作,时间快,效率高,而革兰染色法能提高可疑性感染的阳性检出率。临床检验中可灵活应用两种方法,使报告更加准确。

Application value of routine detection and Gram staining in the detection of vaginal secretions

Objective To explore the application value of routine detection methods (wet slice microscopy and quintuple detection method) and Gram staining method in the detection of vaginal discharge. Methods A total of 479 women attending Jinhua Hospital affiliated to Zhejiang University School of Medicine from January 2021 to December 2021 were selected as study subjects, and vaginal secretion specimens were tested by routine detection method and Gram staining method respectively, and the results were compared and analyzed. Results The Kappa value of quintuple detection method and Gram staining method was 0.793, had a high consistency (P<0.05). In the detection rate of white blood cells, the routine detection method was higher than the Gram staining method, and the difference was statistically significant (P<0.05). In the detection rate of pathogenic bacterium and lactobacillus, Gram staining method was much better than routine detection method, and the difference was statistically significant (P<0.05). Conclusion In daily work, routine detection method can better complete the routine vaginal discharge screening work, the time is fast, the efficiency is high, and the Gram staining method can improve the positive detection rate of suspicious infection. The two methods can be flexibly applied in clinical examination to make the report more accurate.