前列腺素E1对大鼠骨髓间充质干细胞增殖的影响

背景：目前还缺乏前列腺素E1对骨髓间充质干细胞增殖影响的研究。目的：观察前列腺素E1与骨髓间充质干细胞共培养对细胞增殖作用的影响。方法：体外培养SD大鼠骨髓间充质干细胞。取第3 代细胞利用流式细胞仪鉴定细胞表面表型。以每孔1×104数量接种于96 孔板，将前列腺素E1分别以0(对照组)，1，2，5，10，20，40，100 μg/L的浓度作用骨髓间充质干细胞72 h，以CCK-8法检测细胞增殖情况，观察最佳增殖浓度10%的前列腺素E1在不同时间(24，48，72 h)对骨髓间充质干细胞增殖的影响。结果与结论：P3代骨髓间充质干细胞表面阳性率CD11b/c为4.93%，CD29为99.93%，CD45为0.1%，CD90为99.58%，符合骨髓间充质干细胞特征。质量浓度为1，2，5，10，20，40，100 μg/L的前列腺素E1均可以促进骨髓间充质干细胞在体外的增殖，以10 μg/L的作用最强。10 μg/L前列腺素E1在作用48 h后能显著促进大鼠骨髓间充质干细胞增殖(P < 0.05)，其作用呈一定程度的时间依赖性，72 h达最高。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：

Prostaglandin E1 effects on proliferation of rat bone marrow mesenchymal stem cells

BACKGROUND: Currently, there are few studies about prostaglandin E1 on the proliferation of bone marrow mesenchymal stem cells.OBJECTIVE: To explore the effects of prostaglandin E1 on the proliferation of bone marrow mesenchymal stem cells. METHODS: Bone marrow mesenchymal stem cells isolated from Sprague Dawley rats were cultured in vitro. Passage 3 cells were selected to identify cell surface phenotypes by flow cytometry. Cells were seeded onto 96-well microtiter plates at a density of 1×104 cells/well, and then treated with prostaglandin E1 at concentrations of 0, 1, 2, 5, 10, 20, 40, 100 μg/L for 3 days. Cell counting kit-8 assay was applied to assess cellular proliferation, and detect the optimal proliferative concentration of prostaglandin E1 (10%) effects on the proliferation of bone marrow mesenchymal stem at 24, 48 and 72 hours.RESULTS AND CONCLUSION: The positive rates of passage 3 cells were 4.93% for CD11b/c, 99.93% for CD29, 0.1% for CD45, 99.58% for CD90. In vitro proliferation of bone marrow mesenchymal stem cells could be promoted by prostaglandin E1 at concentrations of 1, 2, 5, 10, 20, 40 and 100 μg/L, and the optimal concentration was 10 μg/L. The proliferation of bone marrow mesenchymal stem cells was promoted dramatically after intervention with 10 μg/L prostaglandin E1 for 48 hours (P < 0.05), and peaked at 72 hours in a time-dependent manner.