中药淫羊藿苷干预人工关节磨损微粒诱导的骨溶解

背景：人工关节周围产生骨溶解是关节松动失败的重要原因，各国学者都在寻求一种能有效抑制骨溶解反应的药物，以减少假体松动的发生。目的：观察不同剂量淫羊藿苷干预人工关节磨损微粒诱导骨溶解的效果。方法：将钛合金及骨水泥磨损微粒制成的混悬液分别置入清洁小鼠颅盖骨进行骨溶解建模后，空白组滴入生理盐水到颅盖骨中，药物干预组给予不同浓度的淫羊藿苷(剂量分别为30，60，120 mg/kg)灌胃，每日1次。建模2周后处死小鼠，在显微镜下观察小鼠颅盖骨的结构。经苏木精-伊红染色和免疫组化分析计算破骨细胞数量和小鼠颅盖骨溶解面积的变化。结果与结论：与空白组比较，钛合金微粒和骨水泥微粒建模后的小鼠颅盖骨切片上的骨溶解陷窝数量及破骨细胞数明显增加，图像分析骨溶解陷窝面积也增大(P < 0.05)。淫羊藿苷干预后骨溶解面积减小及破骨细胞数量减少  (P < 0.05)，以120 mg/kg灌胃组最为显著，其次是60 mg/kg，30 mg/kg。结果可见钛磨损微粒和骨水泥磨损微粒能促进破骨细胞的增殖，诱导骨溶解；淫羊藿苷能抑制磨损微粒诱导的破骨细胞形成，从而抑制骨溶解。中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程全文链接：

Icariin intervenes wear particles-induced osteolysis

BACKGROUND:Osteolysis surrounding artificial joints is the main reason for the failure of joint loosening. Scholars all over the world are seeking an effective drug to inhibit osteolysis so as to reduce the occurrence of prosthetic loosening. OBJECTIVE:To observe the effects of different doses of icariin on osteolysis induced by wear particles. METHODS:The wear debris (Ti alloy and bone cement) was put into mouse cranium to create mouse models of calvarial osteolysis. Physiological saline was dropped in the cranium in the blank group. Different concentrations of icariin (30, 60 and 120 mg/kg) were given in the drug intervention group, by intragastric administration, once a day. At 2 weeks after model induction, mice were sacrificed. The structure of mouse cranium was observed under a microscope. Hematoxylin-eosin staining and immunohistochemical staining were performed. The number of osteoclasts and the changes in the calvarial osteolysis area of mice after intervention were calculated. RESULTS AND CONCLUSION:Compared with the blank group, the number of absorption pits of bone slices that were stimulated by wear debris (Ti alloy and bone cement) and the number of osteoclasts were significantly increased, and absorption pits area was also bigger (P < 0.05). After intervention with icariin, osteolysis area and the number of osteoclasts became less (P < 0.05), especially in the 120 mg/kg gavage group, followed by 60 mg/kg and 30 mg/kg. Results suggested that Ti particles and bone cement particles can promote osteoclast proliferation and osteolysis. Icariin can suppress the formation of osteoclasts induced by particles, resulting in inhibiting osteolysis.