瓜蒌皮提取物对人脐带静脉内皮细胞损伤的保护作用

目的研究瓜蒌皮提取物（EPT）对氧化低密度脂蛋白（OX-LDL）诱导的人脐带静脉内皮细胞（HUVECs）损伤的保护作用。方法在培养的人脐带静脉内皮细胞中加入OX-LDL（100mg／L）诱导内皮细胞损伤。检测细胞培养液中非对称性二甲基精氨酸（ADMA）、丙二醛（MDA）、乳酸脱氢酶（LDH）、一氧化氮（NO）、二甲基精氨酸二甲胺水解酶（DDAH）和肿瘤坏死因子-α（TNF-α）的含量。结果体外给予OX-LDL（100mg／L）显著诱导内皮细胞损伤，提高培养液中ADMA、MDA、LDH、和TNF-α水平，降低NO和DDAH水平。两种洗脱（水和70％乙醇洗脱）瓜蒌皮提取物均能显著抑制OX—LDL所致的ADMA、MDA、LDH和TNF-α浓度升高和NO、DDAH降低。结论瓜蒌皮提取物对OX—LDL诱导的人脐带静脉血管内皮细胞损伤有保护作用，其保护作用与降低ADMA和TNF-α浓度有关。

Protective effect of EPT against damages of the human umbilicus vein endothelial cells

Objective To study the protective effect of EPT on damages of the human umbilicus vein endothelial cells （HUVECs） induced by oxidized low-density lipoprotein （ox-LI）L）. Methods The injury of endothelial cells was induced by ox-LDL （100mg/L） in cultured endothelial cells. The medium samples were collected from cultured endothelial cells. And the concentrations of asymmetric dimethylarginine （ADMA）, malondi- aldehyde （MDA）, lactate dehydrogenase （LDH）, nitric oxide （NO）, dimethylarginine dimethylaminohydrolase （DDAH） and turnout necrosis factor-α （TNF-α） were measured. Results Ox-LDL （100 mg/L） significantly increased the concentrations of ADMA, MDA, LDH and TNF-α, and decreased the concentrations of NO and DDAH. EPT with the different elution （water and 70% alcohol） markedly reduced the injury of endothelial cells by ox-LDL, and inhibited the increase of ADMA, MDA, LDH and TNF-α, and inhibited the decrease of NO and DDAH by ox-LDL. Conclusion EPT protects the endothelial cells against damages induced by ox-LDL, and the effects is related to the reduction of ADMA and TNF-α concentration in cultured endothelial cells.