| 靶向survivin基因的RNAi诱导喉癌Hep-2细胞凋亡的研究 |
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| 引用本文： | 李红,谢睿,杨桦,镡旭民. 靶向survivin基因的RNAi诱导喉癌Hep-2细胞凋亡的研究[J]. 山东大学学报(医学版), 2009, 47(8): 58-61 |
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| 作者姓名： | 李红 谢睿 杨桦 镡旭民 |
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| 作者单位： | 第三军医大学附属新桥医院耳鼻咽喉头颈外科,重庆,400037;第三军医大学附属新桥医院耳鼻咽喉头颈外科,重庆,400037;第三军医大学附属新桥医院耳鼻咽喉头颈外科,重庆,400037;第三军医大学附属新桥医院耳鼻咽喉头颈外科,重庆,400037 |
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| 基金项目： | 国家自然科学基金资助(No.30672299) |
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| 摘 要： | 目的通过构建RNAi表达载体,转染喉癌细胞株Hep 2,观察其对survivin表达的抑制作用。方法构建针对survivin基因的干扰载体,用脂质体转染喉癌细胞Hep 2,分析干扰载体对survivin表达的抑制情况,检测细胞凋亡。结果survivin在喉癌细胞Hep 2中呈高表达,其基因序列与GenBank序列对比一致,干扰载体pGensil/survivin能抑制survivin的表达,抑制率为68%,并诱导了喉癌细胞的凋亡。结论针对survivin的小分子RNA可以有效抑制喉癌细胞Hep 2中survivin蛋白的表达,并促进喉癌细胞凋亡。
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| 关 键 词： | RNAi喉肿瘤 Survivin基因 细胞凋亡 |
| 收稿时间： | 2009-03-17 |
RNAi of targeted against survivin induced apoptosis of laryngeal carcinoma cell line hep-2 |
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| LI Hong,XIE Rui,YANG Hua,CHAN Xu-min. RNAi of targeted against survivin induced apoptosis of laryngeal carcinoma cell line hep-2[J]. Journal of Shandong University:Health Sciences, 2009, 47(8): 58-61 |
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| Authors: | LI Hong XIE Rui YANG Hua CHAN Xu-min |
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| Affiliation: | Department Otorhinolaryngology and Head Neck Surgery, Xinqiao Hospital Affiliated to Third Military Medical University, Chongqing 400037, China |
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| Abstract: | Objective To study the inhibitory effects of RNAi expression vector suppressing expression of survivin and inducing the apoptosis of laryngeal carcinoma cell line Hep-2.Methods RNAi expression vectors targeting survivin were successfully constructed,and then were transfected into Hep-2 cells with liposomes.Expression of survivin mRNA was detected and the rate of cell apoptosis was measured.Results There was a high expression of survivin in Hep-2 cells.The DNA sequence was in accordance with the survivin gen... |
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| Keywords: | RNAi Laryngeal squamous cell cancer Survivin gene Apoptosis |
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