类维生素A对单个核细胞白细胞介素2受体表达及细胞因子分泌的影响

目的：　观察视黄醇、视黄酸、维生素Ａ 醋酸酯三种类维生素Ａ（类ＶＡ）在体外对正常人外周血单个核细胞（ＰＢＭＣ）中淋巴细胞白细胞介素２ 受体（ＩＬ－２Ｒ）表达、白细胞介素２（ＩＬ－２）分泌及单核细胞分泌白细胞介素１（ＩＬ－１）的影响。方法：　分别测定１０－ １０～１０－ ６ｍ ｏｌ／Ｌ浓度范围三种类ＶＡ作用下ＩＬ－２ 受体表达、ＩＬ－１、ＩＬ－２ 含量,再测定、比较三种类ＶＡ在１０－ ９浓度时上述作用大小。结果：　在一定浓度（１０－ １０～１０－ ７ｍ ｏｌ）范围,三种物质能不同程度促进高亲和力ＩＬ－２Ｒ表达和ＩＬ－１、ＩＬ－２ 分泌。结论：　类ＶＡ可能作用于ＰＢＭＣ活化早期,促进其功能,且作用大小与类ＶＡ种类及浓度有关。

EFFECTS OF RETINOIDS ON THE EXPRESSION OF IL-2R AND THE PRODUCTION OF CYTOKINES OF PBMC

It is to study the effects of retinoids (retinol, retinoic acid, retinyl acetate) of different concentrations in vitro on expression of high affinity IL 2 receptor, IL 1, IL 2 production of peripheral blood mononuclear cells (PBMC) from normal volunteers. Methods: The changes in binding sites of IL 2 on lymphocyte and the activities of IL 1, IL 2 of each group cultured in the range of 10 -10 -10 -6 mol/L retinoids concentration were measured distinctively. Results: Within certain range of concentration, retinoids could enhance the expression of high affinity IL 2R and the production of IL 1, IL 2. Conclusion: It is suggested that retinoid may enhance the function of PBMC through affecting the early stage in the activation of PBMC. The effects depend on the concentration of retinoids.