C~(13)呼气试验和荧光定量PCR检测幽门螺杆菌的比较

目的比较C~(13)呼气试验和荧光定量PCR在幽门螺杆菌检测中的作用。方法收集106例在我院行胃镜检查患者,取胃粘膜组织用于荧光定量PCR检测幽门螺杆菌,同时对患者进行C~(13)呼气试验检测幽门螺杆菌。结果 C~(13)呼气试验和荧光定量PCR检测幽门螺杆菌阳性率分别为64.2%和67.9%、阴性率分别为35.8%和32.1%,差别无统计学意义(p=0.205);两种检测方法具有较好的相关性(r=0.895,p<0.05)。结论 C~(13)呼气试验和荧光定量PCR检测幽门螺杆菌具有相似的阳性率和阴性率,且具有较好的相关性;但C~(13)呼气试验具有费用低、无创且检测时间短的优点,更值得临床推广。

COMPARISON OF C13 EXPIRATORY TEST AND QRT-PCR DETECTION ON HELICOBACTER PYLORI

Objective To compare the efficacy of C13 expiratory test and qRT - PCR detection on helicohact- er pylori. Methods 106 patients were enrolled and underwent gastric endoscopy examination, and gastric mucous were used for belieobacter pylori detection by qRT - PCR, and patients were also underwent C13 expiratory test for he- lieobacter pylori detection. Results Positive rates of C13 expiratory test and qRT - PCR were 64. 2% and 67. 9% , and negative rates of C13 expiratory test and qRT- PCR were 35.8% and 32. 1% respectively. There were no signifi- cant differences of the positive and negative rates（p = 0. 205 ）. The association between these two methods was well （ r = 0. 895 ,p 〈 0. 05 ）. Conclusion The positive and negative rates of C13 expiratory test and qRT - PCR were simi- lar and the correlation of these two methods was well, however, C13 expiratory test was more deserved to be applied for the clinical use due to its low cost, uninvasive and less detecting time.