Nucelotide sequence of an ECHO virus isolated from a meningiti]

BACKGROUND: To determine the nucleotide sequences of W strain (Zhaoyuan/W/87) isolated from a meningitis patient, and compare them with the sequences of relational enteroviruses. METHODS: Virion RNA was transcribed into cDNA by reverse transcriptase, cDNA was amplified by PCR, the products of PCR were purified. Then RNA sequence analysis was performed by the dideoxynucleotide chain termination method using synthetic oligodeoxynucleotide primers. RESULTS: The partial nucleotide sequences of 5 UTR, region 2C and 3D of W strain (Zhaoyuan/W/87) were respectively 244, 275 and 353 nucleotides long. The complete nucleotide sequences of region VP1 were 876 nucleotides long coding 292 amino acids. In comparison with the VP1 sequence of two ECHO29 strains in Genbank through internet, the homogeneity of nucleotide sequence were 82.1% and 81.7%, that of amino sequence were 95.1% and 94.4%.CONCLUSIONS: W strain Zhaoyuna/W/87) was a mutation strain of ECHO29.