IL-12 IL-18对乙型肝炎患者Th_1/Th_2亚群的影响

目的探讨IL-12I、L-18对乙型肝炎患者Th1/Th2亚群的影响。方法常规分离人外周血单个核细胞(PBMC),在培养基中分别加入IL-12、IL-18和植物血凝素(PHA)培养72 h。用ELISA方法检测上清中IFN-γ、IL-4的含量。结果IL-12联合PHA或IL-18联合HA诱导下,乙型肝炎患者的IFN-γ水平明显高于对照组;IL-12联合PHA诱导下,乙型肝炎患者IL-4水平降低;IL-18联合PHA诱导下轻度患者明显降低;IL-12、IL-18联合PHA诱导下,乙肝患者的IFN-γ水平明显高于对照组,IL-4水平有降低趋势。结论IL-12、IL-18能够诱导乙型肝炎患者PBMC中Th2细胞向Th1细胞转化;而且IL-12的体外作用要强于IL-18。

Affection of IL-12 and IL-18 on Th1/Th2 Subgroup of Hepatitis B

Objective To discuss the affection of IL-12 and IL-18 on Th_1/Th_2 subgroup of hepatitis B patient.Methods The peripheral blood mononuclear cell(PBMC) was isolated routinely from the blood and cultured for 72 hours with adding IL-12,IL-18 and PHA.The IFN-γ、IN-4 contents were detected with ELISA.Results The IFN-γ level in test group under induced by IL-12 combined with PHA or IL-18 combined with PHA was higher than that of in control group significantly and the level of IN-4 decreased in test group.The IFN-γ in combination of inducing with IL-18 with PHA was increased slightly with the tendency decline of IN-4.Conclusion The IL-12、IL-18 might have the ability of tendency to transform the Th_2 cell to the Th_1.The action of IL-12 might be stronger than IL-18 in vitro.