微卫星DNA监控近交系小鼠的重组近交系培育研究

目的采用微卫星DNA技术监控近交系小鼠仔代基因状况，有选择性地进行交配繁殖，以达到快速培育新的重组近交系动物。方法利用PCR扩增微卫星DNA技术对近交系C57和BALB／c小鼠所交配繁殖的仔代鼠进行了微卫星DNA多态性分析，与母代C57小鼠相似系数高的与中的进行定向交配繁殖。结果F2代小鼠多态性位点为14个，纯合基因率为53．3％；到F10代时基因纯合位点达29个，纯合基因位点率为96．7％。每代相似系数具有不断上升的趋势，上升率为3％一10％。采用皮肤移植方法验证了F10代小鼠为近交系。结论建立了一种新的快速培育重组近交系动物的方法，打破了传统近交系的培育方法，为今后研究分析动物间的血缘关系和遗传距离奠定了基础。

Microsatellite DNA Monitoring Cultivation of Recombinant Inbred Strain of Mice

Objective In order to promote the cultivation and establishment of new inbred mice, the heredity genes of baby mice were monitored by microsatellites DNA and selectively breeding in this experiment. Methods DNA polymorphism in baby mice between inbred C57 and BALB/c mice was assessed by microsatellites DNA with PCR amplification. The inbred mice were bred using high and moderate similarity coefficient of female C57 mice of Fo . Results There were 14 polymorphism loci for F2 mice, and rate of homo-gene was 53.3 % . There were 29 homo-gene loci in F,o mice, and rate of homo-gene loci was 96.7 % . The similarity coefficient of mice in the sequential generations was gradually increasing by 3% - 10% from generation to generation. The fact that the mice of F,o generation are inbred mice, was verified and proved by skin transplantation. Conclusion A new method of fast cultivation of inbred mice has been established, therefore, much improved the traditional inbred cultivation techniques. It may considerably facilitate researches on blood relationship and genetic distance in animals in future.