斑秃皮损凋亡因子Annexin V表达及与T细胞浸润的相关性

目的:检测斑秃皮损凋亡因子Annexin V的表达并确定其与T细胞浸润的相关性。方法:对22例斑秃患者皮损(11例早期斑秃和11例中晚期斑秃)和10例正常头皮进行CD3/CD4/CD8和Annexin V的免疫组化双染。结果:早期斑秃Annexin V在浅层血管和毛囊周围的阳性细胞数显著高于中晚期斑秃,而在深层毛囊周围显著低于中晚期斑秃(P0.05)。早期斑秃深层毛囊周围T细胞浸润与Annexin V阳性细胞数量呈显著正相关,而浅层毛囊周围两者无明显相关性。结论:早期斑秃浅层永久性毛囊细胞凋亡与T细胞浸润无相关性,而深层周期性毛囊周围T细胞浸润是毛囊细胞凋亡的主要原因。

Correlation between the apoptotic factor Annexin V and T lymphocytes infiltration in alopecia areata

Objective:To detect the expression of apoptotic factors Annexin V and to determine the rela-tionship between the expression of apoptotic factors Annexin V and the T lymphocyte infiltration in alopecia areata ( AA) . Methods:Immunohistochemical double-staining of CD3/CD4/CD8 and Annexin V was used to detect the expression of apoptotic factors Annexin V in the lesions of 22 patients with AA ( 11 patients at early stage and 11 at advanced stage) and normal scalp of 10 controls. Results:The number of Annexin V-positive cells around vessels and follicular in the superficial layer of the lesions in early stage was significantly higher than that in advanced stage. However, the number in deep layer around follicular of the lesions was significant-ly lower than that in advanced stage ( P<0.05) . There was a positive correlation between the number of T lym-phocyte and Annexin V positive cells in deep layer and there was no significant correlation in superficial layer. Conclusion: The apoptosis of permanent hair follicle cells in superficial layer was uncorrelated with T lympho-cyte infiltration and the T cell infiltration was the main cause of the apoptosis of cyclical hair follicle cells in deep layer.