清络通痹颗粒对胶原性关节炎大鼠T细胞亚群及滑膜细胞的影响

目的：观察清络通痹颗粒(QLT)对胶原性关节炎大鼠T细胞亚群及滑膜细胞TNF-α,IL-1的影响。方法：建立胶原性关节炎(CIA)大鼠模型,同时设正常对照组．给药自d 7始,持续21 d。采用流式细胞术、酶联免疫吸附(EuSA)法、RT-PCR技术,观察QLT对CIA大鼠外周血T细胞亚群及滑膜细胞分泌TNF-α,IL-1和TNF-αmRNA,IL-1 mRNA表达的影响。结果：QLT 14．4 g·kg~(-1)可明显升高CIA大鼠外周血CD4~+T细胞,降低CD8~+T细胞,升高CD4~+/CD8~+比值(P＜0．05,P＜0．01)；QLT 7．2和14．4 g·kg~(-1)降低滑膜细胞分泌TNF-α,IL-1(P＜0．05,P＜0．01)及TNF-αmRNA,IL-1 mRNA的表达。结论：QLT可调节CIA大鼠T细胞亚群的比例,减少炎性细胞因子的产生,提示这可能是其治疗类风湿关节炎主要作用机制的一个重要方面。

Effects of Qingluotongbi granule on T lymphocyte subsets and TNF-α together IL-1 in synoviocytes of collagen induced arthritis in rats

AIM:To investigate the effects of Qingluotongbi granule(QLT)on T lymphocyte subsets and TNF-αtogether IL-1 in synoviocytes of collagen induced arthritis(CIA)in rats.METHODS:The CIA model was created with collagenⅡand Freund complete adjuvant and normal control group was established simultane- ously.Drugs were given on d 7 persisting for 21 d.On d 28,subsets of T lymphocytes in peripheral blood and TNF-α,IL-1,expressions of TNF-αmRNA and IL-1 mRNA in synoviocytes were detected by flow cytometry, ELSIA,and RT-PCR techniques.RESULTS:QLT 14.4 g·kg~(-1) could increase the amount of CD4~+ T lymphocyte and decreased CD8~+ T lymphocyte in peripheral blood of rats with on increase ratio of CD4~+/CD8~+(P＜0.05,P＜0.01);QLT 7.2,14.4g·kg~(-1) could reduce the levels of IL-1 and TNF-αand inhibit the expressions of TNF-αmRNA and IL-1 mRNA in synoviocytes.CONCLUSION:QLT could modulate the ratio of CD4~+/CD8~+ and re- duce the level of inflammatory cytokines,probably being one of the main mechanisms of QLT.