趋化因子及受体与中国HCV/HIV合并感染相关性的研究

目的：探讨趋化因子自细胞介素8（IL-8）、干扰素诱导蛋白10（IFN-inducible 10-kdaprotein，IP-10）及趋化因子受体CCR5、CXCR3，在丙肝病毒（HCV）单纯感染，艾滋病病毒（HIV）单纯感染和HCV／HIV合并感染过程中的表达及意义。方法：采用流式细胞术，检测HCV感染组（n=21）、HIV感染组（n=14）、HCV／HIV感染组（n=28）及正常对照组（n=30）人外周血CD4^＋T淋巴细胞和CD8^＋T淋巴细胞表面CCR5、CXCR3的表达。ELISA方法检测血清趋化因子IL-8、IP-10含量。结果：HCV感染组、HIV感染组和HCV／HIV合并感染组，血清IP-10水平都明显升高，而在合并感染组水平最高；血清IL-8水平在3组亦明显升高。HIV感染组及HCV／HIV合并感染组CD4^＋T细胞表面CXCR3表达显著降低（P〈0．001），CD8^＋T细胞表面CXCR3表达显著升高（P〈0．001）；HCV感染组CD4^＋及CD8^＋T细胞表面CXCR3表达轻度升高，但差异不显著。HCV感染组及HCV／HIV合并感染组CD4^＋及CD8^＋T细胞表面CCR5表达显著降低（P〈0.001）；HIV感染组CD4^＋及CD8^＋T细胞表面CCR5表达显著升高（P〈0.001）。结论：中国HCV／HIV合并感染患者中，血清IL-8和IP-10水平都明显升高；受体CXCR3在CD4^＋T细胞表面表达降低，而在CD8^＋T细胞表面表达升高；受体CCR5在CD4^＋及CD8^＋T细胞表面表达降低，提示趋化因子及受体与HCV／HIV合并感染密切相关。

The expression of chemokine IL-8 and IP-10 in serum and chemokine receptor CCR5 and CXCR3 from PBMCs of HCV/HIV co-infection patients in China

Objective:To investigate expression and significance of CCR5,CXCR3,IP-10 and IL-8 in HCV mono-infection,HIV mono-infection and HCV/HIV co-infection.Methods:We detected the expression of CCR5 and CXCR3 on CD4~+T cells CD8~+T cells from PBMC by FACS and measured serum concentration of IP-10 and IL-8 by ELISA.Results:The serum concentration of IP-10 increased in HCV,HIV and HCV/HIV co-infection group and it was highest in HCV/HIV co-infection.The serum concentration of IL-8 also increased in those three groups.The expression of CXCR3 on CD4~+T cells from PBMCs decreased significantly,however,the expression of CXCR3 on CD8~+T cells increased significantly in HIV and HCV/HIV co-infection group(P<0.001).The expression of CXCR3 on CD4~+T cells as well as CD8~+T cells from PBMCs increased not significantly in HCV group.The expression of CCR5 on CD4~+ as well as CD8~+T lymphocytes increased significantly in HIV group,in contrast,it decreased significantly both in HCV and HCV/HIV co-infection group.Conclusion:In HCV/HIV co-infection,the concentration of IP-10 and IL-8 both increased significantly;The expression of CXCR3 on CD4~+T lymphocytes decreased,however, it increased on CD8~+T lymphocytes;In contrast,the expression of CCR5 on CD4~+ and CD8~+T lymphocytes decreased.It suggested that chemokine and chemokine receptor were related with HCV/HIV co-infection.