Treg细胞和IDO在HPV感染相关宫颈疾病组织中表达及意义

目的 探讨HPV感染相关宫颈疾病患者宫颈组织中Treg细胞和吲哚胺2,3-二氧酶(IDO)的表达及其临床意义.方法 对194例患者行HPV检测、宫颈TCT检查及宫颈病理学检查,然后按病变程度分为正常宫颈(对照组)、HPV阴性(HPV-)宫颈炎、HPV阳性(HPV+)宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌6组实验组,应用Q-PCR、流式细胞术,检测各组宫颈组织中Treg细胞和吲哚胺2,3-二氧酶(IDO)的表达水平.结果 宫颈癌中Treg细胞占CD4+ T细胞的比例及IDO表达水平高于其他组;CINⅢ、CINⅡ 、CINⅠ及HPV(+)宫颈炎组Treg细胞比例高于HPV(-)宫颈炎组及对照组;CINⅢ及CINⅡ组宫颈组织中IDO mRNA表达水平高于CINⅠ、HPV(+)宫颈炎、HPV(-)宫颈炎组及对照组.结论 高危型HPV感染持续存在,Treg细胞数增加,促进宫颈病变进行性发展;HPV感染的CINⅢ、CINⅡ及宫颈癌组织中,IDO呈过表达;IDO和Treg细胞在宫颈癌免疫逃逸机制中发挥重要作用.

Expression and Significance of Treg Cells and IDO in a Spectrum of HPV-Induced Cervical Lesions

Objective To study the clinical significance of the level of regulatory T（Treg） cells and indoleamine 2,3-dioxygenase（IDO） in a Spectrum of HPV-Induced Cervical Lesions.Methods Human papillomavirus（HPV） testing,thinprep cytologic test（TCT） and cervical biopsy were performed in cervical mucosal from 194 women and then all patients were classified as normal cervices（control subjects）,HPV-negative cervicitis,HPV-positive cervicitis,CINⅠ,CINⅡ,CINⅢ or cervical cancer.Flow cytometry was used to assess the percentages of Treg cells in CD4＋ T cells in the cervical mucosal and quantitative PCR was used to analysis the cervical mRNA expression of IDO.Results Cervical cancer had higher numbers of Treg cells and expressed more IDO than other groups.The percentages of Treg cells in CD4＋ T cells in the cervical mucosal were higher in CINⅢ,CINⅡ,CINⅠ and HPV-positive cervicitis than what in HPV-negative cervicitis and control subjects.The expressions of IDO were significantly higher in CINⅢ and CINⅡ than what in CINⅠ,HPV-positive cervicitis,HPV-negative cervicitis and control subjects.Conclusion The development of HPV-induced cervical Lesions is assoiated with HPV persistenced infection and mucosal enrichment of Treg cells.IDO is over expressed in HPV-Induced CINⅢ,CINⅡ and Cervical cancer.Treg cells and IDO may associated with cervical cancer immune escape mechanisms.