多巴胺受体D1基因多态性与原发性高血压的相关性研究

目的:探讨多巴胺受体D1(DRD1)基因(-48A／G)多态性与原发性高血压的相关性。方法:采用多聚酶链式反应结合限制性内切酶片段长度多态性分析方法检测330例原发性高血压患者(高血压组) 和195例健康人(对照组)DRD1基因(-48A／G)多态性,并对两组人群的血压及各项临床指标进行测定。结果:高血压患者中,DRD1基因(-48A／G)多态性各基因型之间的收缩压、舒张压及平均动脉压有显著差异。其中, AG基因型的诊室舒张压、24h舒张压及24h平均动脉压显著高于AA基因型(P<0．05);GG基因型的诊室收缩压、诊室舒张压、诊室平均动脉压及24h舒张压、24h平均动脉压均显著高于AA基因型(P<0．05);AG基因型与GG基因型之间的收缩压、舒张压及平均动脉压均无显著差异(P>0．05)。结论:北京地区汉族人群中DRD1基因(-48A／G)多态性与原发性高血压明显相关。

Association of Gene Polymorphism of Dopamine Receptor Gene ( DRD1 ) With Essential Hypertension in Han Nationality Patients

Objective:To evaluate the association between gene polymorphism of dopamine receptor gene (DRD1) (-48A/G) with essential hypertension. Methods: Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of dopamine receptor gene (DRD1) (-48A/G) in 330 hypertensive patients (EH) and 195 normotensive controls (NE). The blood pressure and clinical data were also measured. Results: Clinic diastolic blood pressure(cDBP),24 h diastolic blood pressure (DBP),24 h mean blood pressure (MAP) of AG genotype of DRD1 (-48A/G) polymorphism were higher than AA genotype (P <0. 05). Clinic systolic blood pressure (cSBP), cDBP, cMAP and 24 h SBP, 24 h DBP, 24h MAP of GG genotype were significantly higher than AA genotype(P< 0.05). Conclusion: Dopamine receptor gene (DRD1) (-48A/G) polymorphism were related to essential hypertension of the Han nationality in Beijing.