何杰金病中t（14;18）染色体易位与Bcl—2蛋白的表达

利用PCR技术检测了24例何杰金病例中的t（14；18），又利用免疫组织化学方法检测了64例何杰金病例中Bcl－2蛋白的表达，并利用上述两种方法对10例反应性增生淋巴结进行了对照检测，结果30％何杰金病例为t（14；18）阳性，同时在2例有明显滤泡增生的反应性增生淋巴结中也检出了这种易位。因此不能确定何杰金病例中的t（14；18）是否来源于肿瘤细胞。此外发现在仅使用mbr引物的条件下，t（14；18）与Bcl－2蛋白高表达之间并无相互对应的关系。

Chromosomal translocation (14; 18) and over expression of Bcl-2 protein in Hodgkin''s disease

Polymerase chain reaction (PCR) and immunohistochemical staining method were used to detect the t (14; 18) and the over expression of Bcl-2 protein in 24 and 64 cases of Hodgkin's disease respectively, and PCR also was used to detect the translocation in 10 cases of reactive lymphoid hyperplasia as contrast. The results showed that t(14; 18) was present in 30% of Hodgkin's disease and that this translocation was also present in two cases of the reactive lymphoid hyperplasia. It is concluded that the expression of Bcl-2 protein is not specific for the t(14;18) chromosomal translocation, and suggested that the t (14; 18) translocation also occur in non-malignant tissue and not, on its own, lead to malignancy.