首页 | 本学科首页   官方微博 | 高级检索  
     

几种抗原修复方法研究
引用本文:王晋芬 田相义. 几种抗原修复方法研究[J]. 诊断病理学杂志, 1997, 4(1): 34-35
作者姓名:王晋芬 田相义
作者单位:山西省肿瘤医院!太原,030013,山西省卫生厅,山西省肿瘤医院!太原,030013,山西省肿瘤医院!太原,030013,山西省肿瘤医院!太原,030013,山西省肿瘤医院!太原,030013
摘    要:由于常规福尔马林固定和石蜡包理过程造成的抗原封闭与结构改变,限制了一些抗体在免疫组织化学中的应用。为了恢复组织的抗原性,提高免疫组织化学的检测阳性率,我们应用高压锅,微波炉和胰蛋白酶分别对石蜡切片进行处理。结果显示,一些抗体过去只能用于冰冻切片,经前处理后可用于石蜡切片,一些抗体的敏感性增强,经高压锅处理的免疫染色重复性较微波炉处理得好,个别抗体适用于酶消化。总之,在免疫组织化学染色过程中应根据抗原抗体的特性选用适当的前处理方法。

关 键 词:抗原修复  抗体  免疫组化

A study of antigen retrieval
Wang Jinfen, Tian Xiangyi, Cong Juan, et al. A study of antigen retrieval[J]. Chinese Journal of Diagnostic Pathology, 1997, 4(1): 34-35
Authors:Wang Jinfen   Tian Xiangyi   Cong Juan   et al
Abstract:The application of some antibodies always has been problematic due to routine formalin fixation and paraffin embedment. In order to unmask the antigens of tissue sections and increase the incidence of immunohistochemical positive results, pressure cooker, microwave and trypsin were used respectively to pretreat paraffin embedded tissue sections. The results showed that (1) some antibodies used to be applied on frozen sections can be used on paraffin sections after processing of pressure cooker and microwave, (2) the sensitivity of some antibodies has been enhanced, (3) the repetition of staining in sections pre-treated by pressure cooker was better than that pretreated by microwave and a few antibodies were suitable for enzyme digestion. In brief, according to the property of antigens and antibodies, a proper pre-treatment processing method should be selected in the course of immunohistochemistry.
Keywords:Antigen retrieval Antibody Immunohistochemistry
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号