| 白花蛇舌草中槲皮素和山柰素的含量测定 |
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| 引用本文： | 杨琴,耿秋霞,郦红岩,魏福荣. 白花蛇舌草中槲皮素和山柰素的含量测定[J]. 现代中药研究与实践, 2012, 0(2): 61-63 |
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| 作者姓名： | 杨琴 耿秋霞 郦红岩 魏福荣 |
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| 作者单位： | 江苏江大源生态生物科技有限公司;江苏七O七天然制药有限公司;江苏吉贝尔药业有限公司 |
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| 摘 要： | 目的建立高效液相色谱法测定白花蛇舌草中槲皮素和山柰素的含量测定方法。方法色谱柱:Kromasil C18(5μm,250×4.6 mm);流动相:甲醇-0.4%磷酸溶液(60:40);检测波长:360 nm,柱温:30℃。结果槲皮素和山柰素的回收率分别为100.5%、101.2%;线性范围分别为0.032 4~0.810μg、0.039 5~0.948μg。结论结果准确,重复性好,可用于该产品的质量控制。
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| 关 键 词： | 白花蛇舌草 槲皮素 山柰素 含量测定 |
Determination of Quercetin and Kaempferol In Hedyotis diffusa Willd. |
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| YANG Qin,GENG Qiu-xia,LI Hong-yan,WEI Fu-rong. Determination of Quercetin and Kaempferol In Hedyotis diffusa Willd.[J]. Research and Practice on Chinese Medicines, 2012, 0(2): 61-63 |
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| Authors: | YANG Qin GENG Qiu-xia LI Hong-yan WEI Fu-rong |
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| Affiliation: | 1.Jiangsu SNS Natural Pharmaceutical Co.Ltd.,Zhenjiang 212005,China;2.Jiangsu Jiangda yestar biotechnology Co.Ltd.,Zhenjiang 212009,China;3.Jiangsu Jibrier Pharm Co.Ltd.,Zhenjiang 212009,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of Quercetin and Kaempferol in Hedyotis diffusa Willd.Methods HPLC was used to quantitative analysis.The Kromasil C18 column(5μm,250×4.6mm) was used,and mobile phase was composed of methanol-0.4%H3PO4/Water(60:40).Detection wavelength was at 360 nm at 30℃.Results The Quercetin and Kaempferol’s spotting recovery were 100.5%、101.2%.Linear range of methods were 0.032 4~0.810μg、0.039 5~0.948μg,respectively.Conclusion The result was accurate and the reproducibility was good.The method could be used for quality control of Hedyotis diffusa Willd. |
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| Keywords: | Hedyotis diffusa Willd. Quercetin Kaempferol determination |
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