分级机械脑损伤动物模型的建立

建立一新的分级机械脑损伤动物模型。方法采用自行设计的撞击装置，以撞击峰压和形变作外伤参娄，并保持较恒定的撞压时间，观察４０只猫在不同撞击水平下的脑生理，病理变化和脑含水量。结果在低撞击水平（ＰＰ０．４５ｋｇ／ｃｍ＾２，ＤＦ２．５ｍｍ），动物出现轻微脑损伤生理反应，病理改变仅局限于撞压处的脑浅层，脑含水量不增加；在中度撞击水平（ＰＰ０．６５ｋｇ／ｃｍ＾２，ＤＦ３．５ｍｍ），则出现明显脑损伤生理反应，

Animal model of graded mechanical brain injury in cats

Objective To produce a new animal model of graded mechanical brain injury. Methods The cats' brains were injured by a new weight drop device designed by ourselfves. The force acting on the dura with a comparatively constant duration and inducing elastic deformation of the brain was expressed with peak pressure (PP) and deformation (DF).The physiological responses, pathological changes and water content of brain were observed in 40 cats. Results At low level of injury (PP: 0.45 kg/cm 2,DF:2.5 mm),there were mild physiological responses of brain injury, local pathological lesions which were in shallow brain under impact screw , and no increase of water content of brain. At moderate level of injury (PP:0.65 kg/cm 2,DF:3.5 mm),the physiological responses were obvious; the pathological lesions of thalamus , hippocampus, and corpus callosum in deep brain were noted with increased water content of brain in the traumatic hemisphere of brain. At high level of injury (PP:0.85 kg/cm 2,DF:4.5 mm),there were violent physiological responses of traumatic brain injury and the patholgical lesions deepened to brain stem with more extensive supratentorial brain lesion; and the water content of brain increased in both hemispheres. Conclusions The results show that the mechanism of the present model is similar to that of clinical accelerating brain injury and the model founds a model of graded mechanical brain injury by parameter of peak pressure and deformation. It is quite useful for studying the pathophysiological mechanism following various degree of traumatic brain injury and making corresponding therapeutic research.