| 白细胞介素-12对DNA疫苗治疗小鼠丙型肝炎病毒的增强作用 |
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| 引用本文： | 冯志华,周永兴,杜德伟,焦成松,王全楚,贾战生,李谨革. 白细胞介素-12对DNA疫苗治疗小鼠丙型肝炎病毒的增强作用[J]. 中华传染病杂志, 2001, 19(1): 11-14 |
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| 作者姓名： | 冯志华 周永兴 杜德伟 焦成松 王全楚 贾战生 李谨革 |
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| 作者单位： | 第四军医大学唐都医院传染科, |
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| 摘 要： | 目的
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| 关 键 词： | 丙型肝炎病毒 DNA疫苗 白细胞介素－12 小鼠 |
| 修稿时间： | 2000-04-26 |
Enhancing therapeutic effect of DNA vaccine against hepatitis C virus infection by interleukin-12 |
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| FENG Zhihua,ZHOU Yongxing,DU Dewei,et al.. Enhancing therapeutic effect of DNA vaccine against hepatitis C virus infection by interleukin-12[J]. Chinese Journal of Infectious Diseases, 2001, 19(1): 11-14 |
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| Authors: | FENG Zhihua ZHOU Yongxing DU Dewei et al. |
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| Affiliation: | FENG Zhihua,ZHOU Yongxing,DU Dewei,et al. Department of Infectious Diseases,Tangdu Hospital,Fourth Military Medical University,Xian 710038,China |
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| Abstract: | Objective To study the effect of interleukin 12 (IL 12) on the therapeutic value of DNA vaccine as well as its tumorigenicity of SP2/0 C transfected with core (HCV C) gene. Methods The HCV core (HCV C) gene cut from pBRTM HCV1 3011 was inserted into the eukaryotic expressive plasmid pcDNA3. Then the recombinant plasmid pcDNA HCV C was constructed and expressed using Lipofectamine in SP2/0 cells. Detection of C protein was proved by immunofluorescence (IF) test. Lastly, mice were immunized by intradermal injections of SP2/0 cells transfected with HCV C gene. Results The tumorigenicity of SP2/0 HCV C cells was reduced significantly by pcDNA HCV C inoculation as compared with mock vector transfected with SP2/0 cells. Moreover, the survival time of mice inoculated with pcDNA HCV C plasmid combined with IL 12 expressive plasmid was prolonged. Conclusion pcDNA HCV C DNA vaccine combined with IL 12 expressive plasmid might get better therapeutic value in the gene therapy against HCV infection. |
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| Keywords: | Hepatitis C virus Vaccine DNA Interleukin 12 |
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