RP-HPLC法测定人血清中茶碱与多索茶碱的浓度

目的:建立以反相高效液相色谱法同时测定人血清中茶碱、多索茶碱浓度的方法。方法:血清采用15%高氯酸进行沉淀后测定,其中色谱柱为InertsilODS-SP,流动相为甲醇-水(25∶75),检测波长为273nm,流速为1mL·min-1,柱温为30℃,内标为巴比妥钠。结果:茶碱、多索茶碱血清浓度分别在0.24～30.69mg·L-1、0.25～31.83mg·L-1范围内线性关系良好(r均为0.9999),最低定量限分别为0.24、0.25mg·L-1;平均相对回收率分别为99.62%～103.92%、96.26%～102.22%;日内RSD分别为0.44%～1.79%、0.54%～1.68%;日间RSD分别为1.68%～2.15%、0.83%～1.57%。结论:本方法简便、快速、准确、灵敏,可用于临床茶碱、多索茶碱血药浓度监测。

Content Determination of Theophyllinum and Doxofylline in Human Serum by RP-HPLC

OBJECTIVE： To establish an RP-HPLC method for the content determination of theophyllinum and doxofylline in human serum. METHODS： An Inertsil ODS-SP column was used with mobile phase consisted of methanol-water（25 ∶ 75） at flow rate of 1 mL·min-1 and the detection wavelength was 273 nm. The column temperature was 30℃ and barbital sodium was used as internal standard. RESULTS：The linear range of theophyllinum and doxofylline were 0.24～30.69 mg·L-1 and 0.25～31.83 mg·L-1 respectively（r=0.999 9）. The detection limit of theophyllinum and doxofylline were 0.24 mg·L-1 and 0.25 mg·L-1.The average relative recovery rate of theophyllinum and doxofylline were 99.62%～103.92% and 96.26%～102.22%. The intra-day RSD were 0.44%～1.79% and 0.54%～1.68% and inter-day RSD were 1.68%～2.15% and 0.83%～1.57%. CONCLUSION： Established method is simple, rapid, sensitive and accurate for clinical serum concentration monitoring of theophyllinum and doxofylline.