IL-10对实验性肝纤维化大鼠血小板衍生生长因子-AA、BB表达的影响

目的:探讨IL-10干预对肝纤维化大鼠表达血小板衍生生长因子-AA、BB(PDGF-AA、BB)的影响。方法:建立大鼠肝纤维化模型并行IL-10干预。分批从对照组(C组)和CCl_4诱导肝纤维化模型组(M组)及IL-10干预肝纤维化组(T组)中取肝脏组织,采用S-P免疫组织化学方法检测分析PDGF-AA、BB在肝纤维化进程中的表达状况和IL-10干预后不同组大鼠及同组不同阶段大鼠肝组织中的表达结果。结果:成功建立大鼠肝纤维化模型小组肝组织中PDGF-AA、BB阳性表达明显增强,且PDGF-BB阳性表达强度高于PDGF-AA。经Ridit分析,两者在C组与M组间比较阳性表达均有显著性差异(P<0.01);T组与M组间比较PDGF-AA无统计学差异,但T组的PDGF-BB较M组明显减弱;PDGF-BB在肝纤维化组与IL-10干预组不同阶段阳性表达差异均有显著性。结论:PDGF-BB阳性强度随肝纤维化进展表达增强,而抑制PDGF-BB的表达可能是外源性IL-10拮抗肝纤维化的机制之一。

Study on the Effect of Interleukin-10 on Expression of Platelet-derived Growth Factor-AA、BB in Rat Experimental Liver Fibrosis

Purpose: To Investigate the effect of interleukin-10 on expression of platelet - derived growth factor-AA,BB in experimental rat liver fibrosis. Method.s: Animal model of liver fibrosis and IL-10 intervention modei was established and the liver tissues were obtained from normai rats(group N), CCl4 induced liver fibrosis rats(gruup C) and IL-10 pretreated rats(group E) in different stages respectively.The expression of PDGF-AA,BB in different groups and stages was measured and analysed by SP immunohistochemistry. Results: The rat liver fibrosis modei was successfully established. Redit analysis indicated that the positive expression of PDGF-AA , BB in group M increased markedly comparing with the group C( ( P < 0.01) , and the positive expression of PDGF-BB was stronger than that of PDGF-AA; there was no difference of PDGF-AA positive expression between group T and group M, but the positive expression of PDGF-BB in group T was significantly weaker than that in group M at different stages. Conclusion; With the progression of the liver fibrosis, the expression of PDGF-AA,BB increased graduale; the antiflbrotic mechanism of IL-10 partly due to its downregulation on the expression of PDGF-BB.