| 精子DNA损伤对体外受精-胚胎移植结局的影响 |
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| 引用本文： | 李娜,江莉. 精子DNA损伤对体外受精-胚胎移植结局的影响[J]. 广西医学, 2011, 33(3): 257-260 |
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| 作者姓名： | 李娜 江莉 |
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| 作者单位： | 广西医科大学第一附属医院生殖中心,南宁市,530021 |
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| 摘 要： | 目的探讨精子DNA损伤对体外受精-胚胎移植（IVF-ET）结局的影响。方法首次行IVF助孕治疗的不孕夫妇51对,运用精子DNA碎片染色质扩散法（SCD）检测精子DNA完整性,夫妇行IVF助孕。于体外受精后第1天观察受精情况,第3天观察卵裂情况。结果精子DNA断裂指数（DFI）与受精率、受精卵卵裂率、2PN卵裂率均呈显著负相关（P均〈0.05）,与优胚率、2PN受精率无明显相关关系。结论精子DNA损伤影响体外受精的治疗结局。随着精子DFI水平的增加,受精率下降,进而对受精卵卵裂及胚胎的发育产生影响。精子DNA完整性可以作为精液质量的检测指标对体外受精结局进行预测。
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| 关 键 词： | 不育症 精子DNA损伤 辅助生殖技术 体外受精 |
Effect of Sperm DNA Damage on the Outcome of In Vitro Fertilization-embryo Transfer |
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| LI Na,JIANG Li. Effect of Sperm DNA Damage on the Outcome of In Vitro Fertilization-embryo Transfer[J]. Guangxi Medical Journal, 2011, 33(3): 257-260 |
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| Authors: | LI Na JIANG Li |
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| Affiliation: | (Center of Reproductive Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To explore the effect of sperm DNA damage on the outcome of in vitro fertilization-embryo transfer（IVF-ET）.Methods The DNA fragmentation of 51 infertile couples undergoing primary IVF-ET was detected by the sperm chromatin dispersion（SCD）test.The pregnancy rate was observed the first day after IVF-ET,and the transgene situation was observed on the third day.Results The negative correlation of fertilization rate,zygote transgene rate,2PN transgene rate with sperm DNA fracture index（DFI）were found（P0.05）,and but no correlation was found with optimal embryo rate and 2PN transgene rate（P0.05）.Conclusion The damage of sperm DNA has an effect on the outcome of IVF.The fertilization rate of IVF decreases as the DFI of sperms increase,which has an effect on the development of fertilized eggs＇ cleavage and embryos.Sperm DNA integrity may be an objective indicator to predict the effect of IVF. |
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| Keywords: | Infertility Sperm DNA damage Assisted reproductive technology In vitro fertilization |
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