甘草黄酮分离纯化工艺

目的:筛选具有酪氨酸酶抑制作用的甘草黄酮分离、纯化工艺.方法:以甘草总黄酮含量及酪氨酸酶抑制率为指标,对甘草黄酮分离纯化工艺进行优化,同时考察不同分离条件对其酪氨酸酶抑制活性的影响.结果:甘草黄酮的最佳分离纯化工艺为30 ～ 60目聚酰胺,上样液pH 6,料液比(甘草黄酮粗提物-聚酰胺)32.48:1,上样液质量浓度3.215 g·L-1,洗脱剂乙醇体积分数70％,洗脱流速3 mL·min-1·g-1,洗脱剂用量1 BV.结论:采用该优选工艺成本低,收率高,安全,操作简便,适合工业化大生产.

Separation and Purification Process of Total Flavonoids from Glycyrrhiza uralensis

Objective:To select separation and purification technology for total flavonoids from Glycyrrhiza uralensis that have inhibitory action on tyrosinase.Method: With the content of total flavonoids and tyrosinase inhibitory rate as indexes,separation and purification technology of total flavonoids from G.uralensis was optimized,and effect of different separation conditions on tyrosinase inhibitory rate were investigated in the same time.Result: Optimal separation and purification process for total flavonoids was:polyamide was 30~60 mech,pH of sample solution was 6,solid-liquid ratio 32.48∶1(extract of total flavonoids from G.uralensis-polyamide),the concentration of sample solution was 3.215 g·L-1,desorption solvent was 70% ethanol,desorption velocity was 3 mL·min-1·g-1,and elution volume was 1 BV.Conclusion: This optimized technology had advantages of low cost,high yield,safe,easy to operate.It was suitable for industrial production.