| 去甲二氢愈创木酸对SHG-44胶质瘤细胞GFAP基因表达的影响 |
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| 引用本文： | 姚莉,卞修武,陈自强.去甲二氢愈创木酸对SHG-44胶质瘤细胞GFAP基因表达的影响[J].第三军医大学学报,2001,23(3):254-256. |
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| 作者姓名： | 姚莉 卞修武 陈自强 |
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| 作者单位： | 第三军医大学附属西南医院病理学研究所, |
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| 基金项目： | 国家自然科学基金资助项目 !(3 9970 2 68) |
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| 摘 要： | 目的：探讨去甲二氢愈创木酸（NDGA）诱导恶性胶质瘤细胞分化过程中中对GFAP基因表达的影响及其意义。方法：用免疫组织化学和原位杂交方法定性、定量检测人恶性胶质瘤细胞系SHG－44细胞GFAP基因的表达水平。结果：NDGA处理后，瘤细胞GFAP蛋白及mRNA表达水平明显增高（P＜0.01）。结论：NDGA具有诱导恶性胶质瘤细胞GFAP基因表达的作用，推测GFAP基因表达上调可能是NDGA诱导胶质瘤细胞分化的作用机制之一。
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| 关 键 词： | 去甲二氢愈创木酸 胶质瘤 诱导分化 胶质纤维酸性蛋白 |
| 文章编号： | 1000-5404(2001)03-0254-03 |
| 修稿时间： | 2000年10月18 |
Effects of nordihydroguaiaretic acid on the expression of GFAP gene of human malignant glioma cell line SHG-44 |
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| YAO Li,BIAN Xiu-wu,CHEN Zi-qiang.Effects of nordihydroguaiaretic acid on the expression of GFAP gene of human malignant glioma cell line SHG-44[J].Acta Academiae Medicinae Militaris Tertiae,2001,23(3):254-256. |
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| Authors: | YAO Li BIAN Xiu-wu CHEN Zi-qiang |
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| Abstract: | Objective To investigate the expression of glial fibrillary acidic protein (GFAP) gene and its significance in the process of glioma cell differentiation induced by nordihydroguaiaretic acid (NDGA). Methods Immunohistochemistry (IHC) and in situ hybridization were used to detect the expression changes of GFAP protein and GFAP mRNA qualitatively and quantitatively. Results The expression levels of GFAP protein and GFAP mRNA in NDGA treatment group were significantly increased compared with the control group ( P <0.01). Conclusion NDGA could induce GFAP gene in malignant glioma cells and the up regulation of this gene expression might be one of the mechanisms by which NDGA induces glioma differentiation. |
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| Keywords: | nordihydroguaiaretic acid (NDGA) glioma differentiation therapy glial fibrillary acidic protein (GFAP) |
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