SARS相关冠状病毒M基因片段的克隆及其DNA疫苗诱导的体液免疫

目的：构建含有SARS相关冠状病毒(severe acute respiratory syndrome coronavirus，SARS—CoV)M基因片段的核酸疫苗，观察其免疫小鼠后病毒特异性抗体产生情况。方法：将合成的M基因片段克隆到核酸疫苗真核表达载体pVAX1中，免疫小鼠后，用SARS—CoV抗体ELISA检测试剂盒定期检测免疫小鼠血清中抗SARS—CoV的病毒特异性抗体水平。结果：构建了重组核酸疫苗质粒pVCo—M，免疫小鼠后可诱导产生出病毒特异性抗体。结论：以M为抗原基因的DNA疫苗能诱导SARS病毒特异性抗体，为进一步改进或探索同类DNA疫苗提供有益启示。

Cloning of SARS coronavirus M gene and humoral immune reaction induced by its DNA vaccine

Objective:To construct DNA vaccine containing SARS CoV M gene and to observe the production of SARS CoV specific antibodies in mice immunized with the plasmid. Methods: A recombinant DNA vaccine plasmid was constructed by cloning SARS CoV M gene fragment into pVAX1.After mice immunized with the recombinant DNA vaccine, the SARS CoV antibodies in the sera of mice were detected with ELISA kit. Results: A recombinant plasmid pVCo M was constructed which could induce SARS CoV specific antibodies in mice. Conclusion: M gene of SARS CoV can be used as an antigen of DNA vaccine to induce SARS CoV specific antibodies.