PI3Kp110beta和前强啡肽在人肝癌组织中的表达

目的 观察人肝癌中磷脂酰肌醇激酶-3(PI3K)p110beta和前强啡肽的表达.方法 应用半定量逆转录.聚合酶链反应(RT-PCR)方法检测10例人中晚期肝癌及其癌旁正常组织中PI3Kp110beta和前强啡肽mRNA基因的相对表达量.结果 PI3Kp110beta和前强啡肽在中晚期肝癌中的表达高于癌旁正常组织,差异有统计学意义(90%比10%;80%比20%,P<0.05).结论 中晚期肝癌中PI3Kp110beta和前强啡肽处于过度表达状态,参与肝癌的发生、发展.

The expressions of PI3Kp110beta and prodynorphin in human hepatocellular carcinoma

Objective To investigate PI3Kp110beta (phesphatidylinositol 3-kinase,catalytic,be-ta polypeptide) and Prodynorphin protein expression in human hepatocellular cell carcinoma (hHCC).Methods The samples were obtained from 10 cases with hHCC specimens. Semi-quantitative RT-PCR strat-egies were used for analyzing the PI3K family members PI3Kp110beta and Prodynorphin mRNA in I0 sam-pies and adjacent noncanerous tissues. Results The expressions of PI3Kp110beta and Prodynorphin mR-NA in hepatocellular cancer were higher than those in the paraneoplastic tissues (90% vs 10% ;80% vs 20% ,P <0.05). Conclusion PI3Kp110beta and Prodynorphin mRNA expressed more in human hepato-cellular cell carcinoma, they may play important roles in the occurrence and development of hepatocellular cancer.