饮酒对尿2 -硫代噻唑烷-4-羧酸排泄的影响

目的观察饮酒对二硫化碳(CS2)接触者及非接触者尿2-硫代噻唑烷4羧酸(TTCA)排泄的影响。方法(1)男性非接触CS2志愿者10人，一次饮用38。白酒150ml或250ml，高效液相色谱法观察其尿TTCA排泄动态；(2)CS2作业男工152人，非接触者60人，分别收集班末尿和晨尿进行TTCA测定并进行问卷调查；同时进行个体空气采样和CS2浓度气相色谱法测定。结果非接触者一次饮白酒150ml后3h尿TTCA水平达峰值，12h后降至饮前水平(餐前0．5h，饮酒后1、3、12h中位数分别为0．045、0．068、0．099、0．046mg/gCr，n=10)；TTCA水平随饮白酒剂量的增加而增高，饮0、150、250ml白酒者TTCA水平(中位数)分别为0．036、0．064、0．609mg/gCr(n=5，饮后3h)。CS2浓度为≤10．0、10．1～50．0、>50．0mg/m3时，CS2接触者TTCA有随CS2浓度增高而上升的趋势；对照组中饮白酒和啤酒者TTCA水平似高于不饮者，而接触组TTCA水平则随饮酒指数的增加而呈降低趋势。结论大量饮酒可影响尿TTCA水平，在进行CS2生物监测时，应避免在大量饮酒后12h内采集尿样，以避免饮酒对监测结果的干扰作用。

Influence of drinking on urinary excretion of 2-thiothiazolidine-4-carboxylic acid

Objective To study the effect of drinking alcohol or beer on excretion characteristics of urinary 2-thiothiazolidine-4-carboxylic acid(TTCA) in peoples exposed to carbon disulfide( CS2) and non-exposure ones. Methods (1)Ten male non-exposure volunteers drank 150 ml or 250 ml hquor(38°) within half an hour, and the excretion dynamic of urinary TTCA were detected with high performance liquid chromatograph. (2) Questionnarie and urine TTCA test were conducted in 152 male workers(end-shift urine) exposed to CS2 and 60 male control peoples(early morning urine).The ambient air at workplace was collected with active personal air monitoring and measured with a gas chromatograph. Results Urinary TTCA levels in non-exposure volunteers reached a high peak after 3 hours of drinking 150 ml liquor, and 12 hours later returned to the levels bofore drinking, the me dian of TTCA bofore drinking, after drinking 1,3 and 12 hours were 0.045,0.068,0.099 and 0.046 mg/g Cr( n =10) ,respectively.TTCA contents were in accordance with the alcohol consumption, the median of TTCA after 3 hours of drinking 0,125 or 250 ml hquor were 0.036,0.064,0. 609 mg/g Cr( n = 5), respectively. There were in creasing tendency of urine TTCA levels in workers exposed to different concentrations of CS2( ≤10.0 mg/m3, 10.1～50.0 mg/m3, > 50.0 mg/m3). When TTCA values were compared with drinking habit in various exposure situation,TTCA levels in people having drinking habit seemed higher( both liquor or beer) in non-exposure group and lower in exposure group, especially in high concentration group ( > 50.0 mg/m3 ). Conclusion Drinking large quantity of liquor may interfere the levels of urinary TTCA. Thus, in biomonitoring of CS2 the suitable sampling time is important to avoid the bias estimation of CS2 exposure.