实验性胰岛素抵抗综合征大鼠模型

目的:建立胰岛素抵抗综合征大鼠模型.方法:用高脂高糖高盐饲料喂养大鼠八周,观察大鼠饮水量,体重,血压,葡萄糖耐量,血脂,血糖及血胰岛素水平,并算出胰岛素敏感性指数等.结果:与基础饲料组大鼠相比,高脂高糖高盐饲料组大鼠饮水量明显增多(多出47%,P＜0.01);体重明显增加(高出11%,P＜0.01);血压明显升高(高出20%,P＜0.01);葡萄糖耐量明显降低(P＜0.01);血清甘油三酯和胆固醇均明显增高(分别高出49%和57%,二者均P＜0.01);高密度脂蛋白胆固醇明显降低(低出30%,P＜0.05);血糖和胰岛素均明显升高(分别高出12%,P＜0.05和69%,P＜0.01),胰岛素敏感性指数明显降低(P＜0.01).结论:高脂高糖高盐饮食可以造成胰岛素抵抗综合征大鼠模型.

Experimental Syndrome of Insulin Resistance Rat Model

Objective:To establish experimental rat model with syndrome of insulin resistance.Methods:Adult male SD rats were divided into two groups and maintained for 8 wk on high-fat-sugar-salt diet and normal diet respectively.Both groups' water drinding amount,body weight,mean arterial BP,glucose tolerance,plasma TG,TC,and HDL-c,glucose and insulin levels,and insulin sensitivity index (ISI) were measured.Results:Compared with normal feeding,high-fat-sugar-salt feeding significantly increased water drinking amount (by 47%, P <0 01),body weight (by 11%, P <0 01),mean arterial BP (by 20%, P <0 01),the levels of plasma TG,TC,insulin (by 49%,57% and 69% respectively,all P <0 01) and glucose (by12%, P <0 05),and remarkably lowered the plasma HDL-c level (by30%, P <0 05) and the ISI ( P <0 01),and induced significant glucose intolerance.Conclusion:Rats fed diet high in fat,sugar and salt can develop to be animal models of syndrome of insulin resistance.