HPLC法测定超微戊己丸中吴茱萸碱和吴茱萸次碱含量

目的：建立超微戊己丸中吴茱萸碱和吴茱萸次碱的含量测定方法。方法采用Kromasail C18色谱柱（250mm×4.6mm，5μm），流动相为流速1.0mL/min的乙腈-乙腈10%（50：50），测定波长为225nm。结果吴茱萸碱和吴茱萸次碱理论板数分别为2681和2067，Y吴茱萸碱=1.965×10^-7X ＋0.07646,r=0.9999）, Y吴茱萸次碱=3.658×10^-7X-0.2199,r=0.9999）,吴茱萸碱与吴茱萸次碱的线性区间分别为10.2～51.0μg/mL以及10.0～50.0μg/mL ，两者平均回收率分别为97.3%和101.4%，其相对标准偏差分别为3.2%和3.9%，最低检测限为0.05μg/mL和0.1μg/mL。结论使用高效液相色谱法对超微戊己丸当中的吴茱萸碱以及吴茱萸次碱含量进行检测的精确率较高，重现性好。

Measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills based on HPLC method

Objective To establish a method for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills. Methods Chromatographic column： Kromasail C18（250mm×4.6mm, 5μm）; mobile phase：acetonitrile-acetonitrile（50：50）; flow rate： 1.0mL/min; wavelength： 225nm. Results The number of theoretical plates for evodiamine and rutaecarpin was 2681 and 2067 respectively（Yevodiamine=1.965×10^-7X＋0.07646, r=0.9999;Yrutaecarpin=3.658×10^-7X-0.2199, r=0.9999）. Evodiamine and rutaecarpin had linear interval of 10.2-51.0μg/mL and 10.0-50.0μg/mL respectively, average recovery rate of 97.3% and 101.4% respectively, relative standard deviation of 3.2% and 3.9% respectively, lowest detection limit of 0.05μg/mL and 0.1μg/mL respectively. Conclusion High performance liquid chromatography demonstrates high accuracy and good reproducibility for measurement of evodiamine content and rutaecarpin content in ultramicro Wuji pills.