微小 RNA-520g、微小 RNA-532在 80例多发性骨髓瘤骨髓组织中的表达

目的 观察多发性骨髓瘤病人骨髓组织中微小RNA-520g(miR-520g)和微小RNA-532(miR-532)的表达,并探讨其临床意义.方法 选取2015年7月至2019年7月西安市中心医院收治的多发性骨髓瘤病人80例为观察组,同时选取同期进行骨髓穿刺并明确骨髓功能无异常的非血液病病人80例为对照组.采用实时荧光定量PCR(qRT-PCR)法检测两组骨髓组织中miR-520g、miR-532的表达水平;分析两者与多发性骨髓瘤临床病理特征的关系;采用Pearson法分析miR-520g、miR-532表达的相关性;通过受试者工作特征曲线(ROC)检测miR-520g、miR-532的表达对多发性骨髓瘤的诊断价值.结果 观察组病人骨髓组织中miR-520g表达水平(3.70±0.70)均显著高于对照组(2.79±0.65),观察组miR-532表达水平(0.90±0.24)显著低于对照组(1.32±0.31)(P<0.05).观察组病人骨髓组织中miR-520g、miR-532表达水平与病人临床分期、分化程度、是否发生淋巴结转移相关(P<0.05).ROC结果显示miR-520g诊断多发性骨髓瘤曲线下面积(AUC)为0.827,敏感度为73.8％,特异性为80.0％,miR-532诊断多发性骨髓瘤的AUC为0.855,敏感度为82.5％,特异性为75.0％,二者联合诊断多发性骨髓瘤的AUC为0.922,敏感度为81.3％,特异性为91.2％.结论 多发性骨髓瘤病人骨髓组织中miR-520g呈高表达,miR-532呈低表达,两者呈负相关,且与临床分期、分化程度、是否发生淋巴结转移相关,骨髓组织miR-520g、miR-532表达对多发性骨髓瘤具有一定的诊断价值.

Expressions of miR-520g and miR-532 in bone marrow of patients with multiple myeloma: an analysis of 80 cases

Objective To observe the expressions of microRNA-520g (miR-520g) and microRNA-532 (miR-532) in bone marrow of patients with multiple myeloma and to explore their clinical significance.Methods Eighty patients with multiple myeloma admitted toXi''an Central Hospital from July 2015 to July 2019 were selected as the observation group, and another 80 patients who had bone marrow puncture at the same time and found no abnormal bone marrow function were selected as the control group. The expression levels ofmiR-520g and miR-532 in bone marrow tissues of the two groups were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationships between miR-520g, miR-532 and the clinicopathological features of multiple myeloma were analyzed.Pearson method was used to analyze the correlation of miR-520g and miR-532 expressions, and the diagnostic value of miR-520g and miR-532 in multiple myeloma was detected by receiver operating characteristic (ROC) curve.Results The expression level of miR520g in the bone marrow tissues of the observation group was significantly higher than that of the control group (3.70±0.70) vs. (2.79± 0.65)], and the expression of miR-532 was significantly lower than that of the control group (0.90±0.24) vs. (1.32±0.31), P<0.05]. The expression levels of miR-520g and miR-532 in the bone marrow tissues of the patients in the observation group were correlated with theclinical stage, differentiation degree and lymph node metastasis (P<0.05). ROC curve analysis results showed that the area under the curve (AUC) of miR-520g in the diagnosis of multiple myeloma was 0.827, the sensitivity was 73.8%, and the specificity was 80.0%. The AUC of miR-532 in the diagnosis of multiple myeloma was 0.855, the sensitivity was 82.5%, and the specificity was 75.0%. The AUC of miR-520g and miR-532 in the diagnosis of multiple myeloma was 0.922, the sensitivity was 81.3%, and the specificity was 91.2%.Conclusions MiR-520g is highly expressed and miR-532 is lowly expressed in the bone marrow tissues of patients with multiple myeloma. Their expressions are negatively correlated, and related to clinical stage, degree of differentiation and lymph node metastasis. The expressions of miR-520g and miR-532 in bone marrow have certain diagnostic value for multiple myeloma.