抗中性粒细胞胞浆抗体与抗核抗体联合检测在系统性红斑狼疮中的临床意义

目的:探讨系统性红斑狼疮(SLE)患者血清中抗中性粒细胞胞浆抗体(ANCA)及抗核抗体(ANA)联合检测的临床意义。方法:采用间接免疫荧光技术(IIF)检测并回顾性分析84例SLE患者和52例健康体检者(正常对照组)血清ANCA阳性率及荧光免疫模型,同时采用酶标免疫吸附法(ELISA)进行抗核抗体(ANA)检测。结果:84例SLE患者中,ANCA阳性28例(33.3%),正常对照组无ANCA阳性。28例AN-CA阳性组中,狼疮活动18例(64%),56例ANCA阴性组中狼疮活动22例(39.3%),两组阳性率差别有统计学意义(P<0.01);84例SLE患者中ANA检出58例(69.0%),正常对照组52例中2例ANA阳性,阳性率为(3.84%)。ANCA与ANA联合检测,ANCA阳性ANA阳性共70例,敏感性为83.3%,显著高于两者单独检测时的敏感性(P<0.01)。结论:联合ANCA、ANA检测有助于提高SLE的诊断的敏感性,ANCA与狼疮活动力具有相关性。

Clinical significance of serum ANCA combining with ANA tests in patients with systemic lupus erythematosus

Objective:To study the clinical significance of antineutrophil cytoplasmic antibody(ANCA) combining with antinuclear antibody(ANA) tests in patients with systemic lupus erythematosus.Methods:The spectrums of ANCA in SLE group(n=84) and healthy control group(n=52) were tested by indirect immune fluorescence(IIF),and ANA were detected by enzyme linked immuno sorbent assay(ELISA).Results:In SLE group,28 cases were ANCA positive with the positive rate of 33.3%,and no ANCA positive case was found in the healthy control group.18 of 28 ANCA positive cases were lupus activity(64%),and 22 of 56 ANCA negative cases were lupus activity(39.3%),the positive rates between the two groups had statistical significance(P<0.01).58 of 84 cases with systemic lupus erythematosus were ANA positive(69.0%).In healthy control group,there were 2 cases with ANA positive(3.84%).With ANCA and ANA joint detection,ANCA positive and ANA positive were 70 cases in the 84 patients with systemic lupus erythematosus,the sensitivity was 83.3%,significantly higher than that of separate detection(P<0.01).Conclusion:The joint detection of ANA and ANCA is helpful for the diagnosis of systemic lupus erythematosus,and ANCA had correlation with lupus activity.