卫氏并殖吸虫后尾蚴膜超微结构和后尾蚴膜反应的免疫组织化学定位

本文应用透射电镜和免疫组织化学技术观察后尾蚴膜的超微结构,确定反应活性部位.后尾蚴在兔抗血清中孵育3h后,体表有反应膜形成,皮层结构出现损伤现象,免疫组织化学研究表明抗原抗体复合物结合于皮层表面。初步推测,后尾蚴膜反应是抗原抗体在虫体表面发生沉淀反应的结果,反应定位于皮层表面,抗原可能来自后尾蚴分泌排泄物。

ULTRASTRUCTURE OF METACERCARIAL MEMBRANE AND IMMUNOHISTOCHEMICAL LOCALIZATION OF METACERCARIAL MEMBRANE REACTION OF PARAGONIMUS WESTERMANI

The ultrastructure of the tegument of the early excysted P. westermani metacercariae Was observed with transmission electron microscope. The tegument was fine and dense in texture, containing a lot of disk-like bodies and a few mitochondria. The outer-plasma membrane was clear and intact.After the metacercariae were incubated in rabbit anti-serum for 3 hours, metacereanal membrane reaction (MMR) appeared closely to the outer plasma membrane and the tegument was damaged.Indirect peroxidase-labelled immunohistochemical technique was used to identify the location of the MMR at ultrastructural level. The result showed that the antibody-antigen complex was localized on the tegumental surface of the worm. The data constituted the evidence that the MMR resulted from the antibody-antigen reaction and the tegumental surface was the reaction site for the MMR (Figs. 1-4).