| 栀子药材的HPLC指纹图谱研究 |
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| 引用本文： | 任淑娟,孙辉,丁野,李文莉. 栀子药材的HPLC指纹图谱研究[J]. 华西药学杂志, 2012, 27(3): 309-311 |
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| 作者姓名： | 任淑娟 孙辉 丁野 李文莉 |
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| 作者单位： | 任淑娟 (湖南中医药大学,湖南长沙410208) ； 孙辉 (湖南省食品药品检验研究院,湖南长沙410001) ； 丁野 (湖南省食品药品检验研究院,湖南长沙,410001) ； 李文莉 (湖南省食品药品检验研究院,湖南长沙,410001) ； |
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| 基金项目： | 国家“十一五”重大科技支撑计划“重大新药创制”课题 |
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| 摘 要： | 目的建立栀子药材的HPLC特征指纹图谱,为其质量控制提供依据。方法采用RP-HPLC法,色谱柱为Phenome-nex C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水线性梯度洗脱,检测波长265 nm,流速0.8 mL·min-1,柱温35℃,并进行数字化评价。结果建立了栀子HPLC指纹图谱分析的研究方法,在指纹图谱中共有峰9个,并指认了3个色谱峰,指纹图谱的相似度均在0.98以上。结论建立的栀子药材HPLC指纹图谱可对栀子药材中的化学成分信息进行全面反映,适用于栀子药材的规范化种植及质量控制。
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| 关 键 词： | 栀子 高效液相色谱法 指纹图谱 质量控制 |
HPLC fingerprint of Gardeniae fructus |
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| REN Shu-juan,SUN Hui,DIN Ye,LI Wen-li. HPLC fingerprint of Gardeniae fructus[J]. West China Journal of Pharmaceutical Sciences, 2012, 27(3): 309-311 |
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| Authors: | REN Shu-juan SUN Hui DIN Ye LI Wen-li |
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| Affiliation: | 1.Hunan University of Chinese Medicine,Changsha,Hunan,410208 P.R.China;2.Hunan Provincial Institute for Drug Control,Changsha,Hunan,410001 P.R.China) |
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| Abstract: | OBJECTIVE To establish the HPLC fingerprint of Gardeniae fructus,for its quality control.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.Chromatographic condition:Phenomenex-column,gradient elution with acetonitrile-water,the detection wavelength was at 265 nm,flow rate was at 0.8 mL·min-1,and the column temperature was 35 ℃.RESULTS HPLC-fingerprint of Gardeniae fructus from different areas was established,which contained 9 common peaks,and 3 peaks in the fingerprint were identified,and the similarities were over 98%.CONCLUSION The HPLC chromatographic fingerprint of Gardeniae fructus can express the chemical information completely and can be used for the standardization implantation and quality control of Gardeniae fructus. |
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| Keywords: | Gardeniae fructus HPLC Fingerprint Quality control |
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