壳聚糖纳米粒Zeta电位与载药量关系初探

目的以壳聚糖作为载体材料、冬凌草甲素为模型药物,制备载药纳米粒,研究载药纳米粒Zeta电位与载药量的关系。方法采用离子交联法在系列pH下制备出不同Zeta电位的冬凌草甲素-壳聚糖纳米粒(Ori-CS-NPs)。测量粒径分布、多分散性和Zeta电位,用HPLC测定载药量,对数据进行回归分析。结果初步得出了ORI-CS-NPs(粒径242.01±11.45nm,PDI<0.3)的Zeta电位随pH升高而降低,载药量随Zeta电位的增高而降低。结论采用离子交联法在不同pH下可制备出粒径分布均匀、Zeta电位及载药量呈一定规律变化的载药纳米粒;纳米粒的Zeta电位与载药量呈线性关系。

Preliminary study on the relationship between Zeta potential and drug loading of chitosan nanoparticles

OBJECTIVE Choose chitosan as the carrier and oridonin as the model drug to prepare drug-loaded nanoparticles and study the relationship between their zeta potential and drug loading officielly.METHODS The oridonin-chitosan-nanoparticles(Ori-CS-NPs) with different zeta potential were prepared by ionic cross-linking method at different pH.Their size,polydispersity and Zeta potential(ZP) were determined.The drug loading(DL%) was determined by HPLC.The data was analyzed by regression,and the relationship between ZP and DL% was primarily obtained.RESULTS The diameter of Ori-CS-NPs was 242.01±11.45 nm with polydispersity less than 0.3 and the Zeta potential decreased with increasing pH while drug loading decreased with increasing Zeta potential.CONCLUSION The CS-NPs can be prepared by ionic cross-linking method with good size distribution.The equation of regression indicated a linear relationship between the zeta potential and the drug load.