| 夏枯草花粉离体萌发观察 |
| |
| 引用本文： | 李义敏,万德乾,郭巧生,谢越盛,周曼. 夏枯草花粉离体萌发观察[J]. 中国中药杂志, 2013, 38(9): 1340-1343 |
| |
| 作者姓名： | 李义敏 万德乾 郭巧生 谢越盛 周曼 |
| |
| 作者单位： | 南京农业大学中药材研究所,江苏南京,210095 |
| |
| 基金项目： | 国家自然科学基金项目(30772730,81072986);国家大学生创新性试验计划项目(111030722);南京农业大学SRT计划项目(114A29) |
| |
| 摘 要： | 选用唇形科植物夏枯草花粉为材料,通过在离体条件下花粉的萌发来探讨夏枯草花粉萌发的最适条件.以10％ H3BO4,30％ Ca(NO3)2,20％ MgSO4,10％ KNO3配制成无机盐溶液为基本培养基,统计不同相对分子质量的PEG,不同浓度的蔗糖,不同pH和不同温度条件以及不同采集时间下花粉萌发率的情况.夏枯草花粉萌发的最适液体培养条件是:10％ H3BO4 +30％ Ca(NO3)2 +20％ MgSO4+10％ KNO3+25％ PEG-4000,pH在6.5左右,刚刚开花时采集的花粉在此条件下花粉萌发率最高.
|
| 关 键 词： | 夏枯草 花粉 离体萌发条件 |
| 收稿时间： | 2012-08-31 |
Observations of in vitro pollen germination of Prunella vulgaris |
| |
| LI Yi-min,WAN De-qian,GUO Qiao-sheng,XIE Yue-sheng and ZHOU Man. Observations of in vitro pollen germination of Prunella vulgaris[J]. China Journal of Chinese Materia Medica, 2013, 38(9): 1340-1343 |
| |
| Authors: | LI Yi-min WAN De-qian GUO Qiao-sheng XIE Yue-sheng ZHOU Man |
| |
| Affiliation: | Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China;Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China |
| |
| Abstract: | To determine the optimal condition of pollen germination.The pollen of Prunella vulgaris was cultured in vitro.Pollen germination rates were recorded using 10% H3BO4, 30% Ca(NO3)2, 20% MgSO4 and 10% KNO3 as the basic mineral medium with PEG of different molecular weight, sucrose of various density and multiple pH value.The rates were also measured under different cultivation temperature and pollen acquisition time.The optimal condition of pollen germination is 10% H3BO4, 30% Ca(NO3)2, 20% MgSO4, 10% KNO3, and 25% PEG-4000 as the medium, with pH about 6.5 and pollen acquired at the beginning of blossom. |
| |
| Keywords: | Prunella vulgaris pollen condition of in vitro germination |
| 本文献已被 万方数据 等数据库收录！ |
|
