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| 地黄连作障碍中钙信号系统的异常变化分析 | |
| 引用本文： | 郭冠瑛,李明杰,王鹏飞,王丰青,何华勤,李娟,郑红艳,陈新建,张重义.地黄连作障碍中钙信号系统的异常变化分析[J].中国中药杂志,2013,38(10):1471-1478. |
| 作者姓名： | 郭冠瑛 李明杰 王鹏飞 王丰青 何华勤 李娟 郑红艳 陈新建 张重义 |
| 作者单位： | 1. 河南农业大学农学院,河南郑州450002;福建农林大学中药材GAP研究所,福建福州350002 2. 河南农业大学农学院,河南郑州,450002 3. 福建农林大学中药材GAP研究所,福建福州,350002 4. 福建农林大学中药材GAP研究所,福建福州350002;河南农业大学农学院,河南郑州450002 |
| 基金项目： | 国家自然科学基金项目(30973875,81072983,81274022,31271674) |
| 摘 要： | 目的:阐述钙信号系统相关基因在头茬、重茬地黄中不同时期、不同组织中的相对表达量；同时利用不同浓度的钙信号阻断剂(异搏定、肝素钠)处理重茬地黄,进一步验证钙信号系统在连作伤害中的作用方式.方法:在前期转录组和连作地黄数字差异表达谱文库构建基础上,筛选鉴定了在连作地黄中差异表达的钙信号体系相关蛋白基因,并利用实时荧光定量PCR技术对钙信号系统相关基因进行时空表达分析；利用不同浓度的钙信号阻断剂(异搏定、肝素钠)处理重茬地黄.结果:12个基因中2个背向细胞质的钙离子通道在重茬地黄中下调表达,10个朝向细胞质的钙离子通道在重茬地黄中上调表达；而施用钙信号阻断剂的重茬地黄中,钙信号响应元件的CBL,CBP,CIBP,PLC等关键基因表达均显著下调,植株的受害程度显著缓解,并接近头茬水平.结论:连作伤害引起了钙由内质网向细胞质中流动,钙信号系统参与了地黄连作毒害的感知、传导和放大过程. |
| 关 键 词： | 地黄 连作障碍 钙信号系统 荧光定量PCR 钙信号阻断剂 |
| 收稿时间： | 2012/9/26 0:00:00 |
Abnormal change of calcium signal system on consecutive monoculture problem of Rehmannia glutinosa |
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| GUO Guan-ying,LI Ming-jie,WANG Peng-fei,WANG Feng-qing,HE Hua-qin,LI Juan,ZHENG Hong-yan,CHEN Xin-jian and ZHANG Zhong-yi.Abnormal change of calcium signal system on consecutive monoculture problem of Rehmannia glutinosa[J].China Journal of Chinese Materia Medica,2013,38(10):1471-1478. | |
| Authors: | GUO Guan-ying LI Ming-jie WANG Peng-fei WANG Feng-qing HE Hua-qin LI Juan ZHENG Hong-yan CHEN Xin-jian and ZHANG Zhong-yi |
| Institution: | School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;Institute of Chinese herbal medicine GAP, Fujian Agriculture and Forestry University, Fuzhou 350002, China;School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;Institute of Chinese herbal medicine GAP, Fujian Agriculture and Forestry University, Fuzhou 350002, China;School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;Institute of Chinese herbal medicine GAP, Fujian Agriculture and Forestry University, Fuzhou 350002, China;School of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;Institute of Chinese herbal medicine GAP, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaSchool of Agronomy, Henan Agricultural University, Zhengzhou 450002, China |
| Abstract: | |
| Keywords: | Rehmannia glutinosa succession cropping obstacles calcium signal system the real-time quantitative PCR calcium signal blocking agents |
| 本文献已被 万方数据 等数据库收录！ | |