| HPLC法测定复方银连颗粒中绿原酸的含量 |
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| 引用本文： | 夏莲. HPLC法测定复方银连颗粒中绿原酸的含量[J]. 中国药房, 2011, 0(31): 2943-2944 |
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| 作者姓名： | 夏莲 |
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| 作者单位： | 广西北海食品药品检验所; |
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| 摘 要： | 目的:建立测定复方银连颗粒中绿原酸含量的方法。方法:采用高效液相色谱法。色谱柱为Welchrom C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(10∶90),检测波长为327nm;流速为1.0mL·min-1,柱温为30℃。结果:绿原酸进样量在0.3035～1.5175μg范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为101.6%,RSD=1.84%(n=6)。结论:本方法操作简便、可靠,可用于复方银连颗粒的质量控制。
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| 关 键 词： | 复方银连颗粒 高效液相色谱法 绿原酸 含量测定 |
Content Determination of Chlorogenic Acid in Compound Yinlian Granules by HPLC |
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| XIA Lian. Content Determination of Chlorogenic Acid in Compound Yinlian Granules by HPLC[J]. China Pharmacy, 2011, 0(31): 2943-2944 |
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| Authors: | XIA Lian |
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| Affiliation: | XIA Lian(Beihai Institute for Food and Drug Control of Guangxi,Beihai 536000,China) |
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| Abstract: | OBJECTIVE：To establish the method for the content determine of chlorogenic acid in Compound yinlian granules.METHODS：HPLC method was adopted.The Welchrom C18 column（250 mm×4.6 mm,5 μm）was used with mobile phase consisted of acetonitrile-0.1% phosphoric acid（10：90）.The detection wavelength was set at 327 nm and flow rate was 1.0 mL·min-1.The column tempareture was 30 ℃.RESULTS：The calibration curve of baicalin was linear within the range of 0.303 5～1.517 5 μg（r=0.999 6）.The average recovery was 101.6%（RSD=1.84%,n=6）.CONCLUSION：The method is simple,convenient and reliable.It is appropriate for the quality control of Compound yinlian granules. |
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| Keywords: | Compound yinlian granules HPLC Chlorogenic acid Content determination |
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