rhBMP-2对人牙周膜细胞骨桥蛋白表达的影响

目的　深入了解牙周膜细胞 (periodontalligamentcells,PDLC)的成骨样细胞特性 ,以及非胶原蛋白在牙周组织矿化中的作用。方法　在体外培养条件下 ,观察重组人骨形成蛋白 2(recombinanthumanbonemorphogeneticprotein 2 ,rhBMP 2 )作用前后是否对矿化相关蛋白之一的骨桥蛋白 (osteopontin ,OPN)在人PDLC中的存在及表达情况产生影响。在小玻片上培养第 5代PDLC ,分为加rhBMP 2 (5 0 μg/L)刺激的实验组和不加任何因子的空白对照组 ,以地高辛标记的OPNcDNA探针 ,采用原位杂交技术对PDLC中OPN的表达情况进行检测 ;同时做PBS替代探针和RNA酶预处理的方法学对照。结果　对照组、替代对照和RNA酶预处理的PDLC均显示为阴性反应 ,没有显示出OPN的阳性表达信号 ;实验组PDLC的胞浆中可见明显的OPN阳性反应 ,表达信号较强。结论　rhBMP 2的刺激作用可使PDLC表达出较强的OPN阳性信号 ;表明在一定条件和外界因子的诱导下 ,PDLC具有向成骨特性方向转变的潜能 ,对牙周组织再生有促进效应。

Effect of recombinant human bone morphogenetic protein-2 on the expression of osteopontin in human periodontal ligament cells

Objective To investigate the effects of growth factor rhBMP-2 on the expression of osteopontin in periodontal ligament cells(PDLC). Methods In culture model of human PDLC, in situ hybridization method was used to detect the expression of osteopontin in human PDLC. Results The PDLC showed negative reaction to express osteopontin, while those PDLC stimulated by rhBMP-2 showed osteopontin mRNA positive reaction in plasm. Conclusion rhBMP-2 can induce the expression of osteopontin in PDLC. rhBMP-2 may be an effective growth factor in stimulating PDLC differentiation to osteoblast and promote periodontal regeneration.