Development of an enzyme‐linked immunosorbent assay (ELISA) for the detection of porcine pepsin as a milk clotting enzyme |
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| Authors: | M. El‐Batawy A. S. Zahran S. A. Madkor C. J. Smith |
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| Affiliation: | 1. Faculty of Agriculture , University of Cairo , Fayoum, Egypt;2. Faculty of Agriculture , University of El‐Minia , Egypt;3. The North East Wales Institute , Connah's Quay, Clwyd, CHS 4BR, UK |
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| Abstract: | An enzyme‐linked immunosorbent assay (ELISA) for the detection of porcine pepsin in milk‐clotting enzyme preparations has been developed. The assay is capable of detecting porcine pepsin in the range 1 μgto 1 mg ml‐1 without enhancement or modification. The specificity of the technique was studied by inhibition assay. Slight cross‐reactions with bovine rennet and Mucor miehei rennet occurred at high concentrations (1.0 mg ml‐1). The ELISA used in this investigation appears to provide a quick, sensitive and specific method for the detection of porcine pepsin and has potential applications in the dairy industry. |
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| Keywords: | Porcine pepsin milk rennet |
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