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| 利用RCCS体外快速构建组织工程化骨的实验研究 | |
| 引用本文： | 何东宁,吕春堂,金岩. 利用RCCS体外快速构建组织工程化骨的实验研究[J]. 口腔颌面外科杂志, 2005, 15(1): 18-20 |
| 作者姓名： | 何东宁 吕春堂 金岩 |
| 作者单位： | 1. 第二军医大学长海医院口腔科,上海,200433 2. 第四军医大学口腔医院组织工程中心,西安,710032 |
| 摘 要： | 目的:探讨利用旋转式细胞培养系统(RCCS)在体外快速构建组织工程骨的可行性。方法:将骨髓基质细胞接种于煅烧骨鄄胶原支架上,分别在RCCS和静态环境中进行培养,8、24和72h后取材,细胞计数,检测碱性磷酸酶活性,扫描电镜观察成骨潜能。结果:随培养时间延长,细胞增殖和碱性磷酸酶活性均有所增长,但RCCS组明显高于静态培养组。超微结构观察,可见有颗粒状分泌物和丝状胶原纤维,形成三维结构。结论: 旋转式细胞培养系统是体外快速构建组织工程化骨的理想方法。 |
| 关 键 词： | 组织工程 体外培养 旋转式细胞培养系统 |
| 文章编号： | 1005-4979(2005)01-0018-03 |
| 修稿时间： | 2004-09-15 |
Experimental Study on Rapid Construction of Tissue Engineered Bone in vitro by Rotary Cell Culture System |
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| HE Dong-ning,LV Chun-tang,JIN Yan. Experimental Study on Rapid Construction of Tissue Engineered Bone in vitro by Rotary Cell Culture System[J]. Chinese Journal of Oral and Maxillofacial Surgery, 2005, 15(1): 18-20 | |
| Authors: | HE Dong-ning LV Chun-tang JIN Yan |
| Affiliation: | HE Dong-ning1,LV Chun-tang1,JIN Yan2 |
| Abstract: | Objective: To explore the possibility of rapid construction of tissue engineered bone in vitro by RCCS. Methods: MSCs were seeded on CBB-collagen , and then cultured in RCCS and static environment respectively. 8, 24 and 72 hours after set up, the osteogenic potential of the cell-scaffold constructs were investigated by cell counting, alkaline phosphatase(ALP) activity tests, and ultrastructural observation under scanning electron microscope. Results: With the increase of culture time, the proliferation and ALP activity of cell all increased. But the increase of RCCS group were significantly higher than those of static group. Ultrastructural investigation revealed that marrow stromal stem cells produce collagen fibrils and form 3-dimensional extracellar matrix. Conclusion: RCCS may be an ideal method for rapid construction of tissue engineered bone in vitro. |
| Keywords: | tissue engineering culture in vitro rotary cell culture system |
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