GX-50抗皮肤衰老的研究

目的 研究GX-50抗皮肤衰老的作用。方法 通过在20月高龄小鼠背部涂抹GX-50，采用HE染色和免疫组织化学法对GX-50抗皮肤衰老的作用进行组织学观察。原代培养人皮肤成纤维细胞，采用H₂O₂诱导建立皮肤细胞衰老模型，分为空白对照组、DMSO组、GX-50组、H₂O₂组和GX-50预处理后H₂O₂处理组（GX-50+H₂O₂组）；采用β-半乳糖苷酶染色实验以及测定细胞分泌物丙二醛（MDA）和细胞内活性氧（ROS）含量检测GX-50在细胞水平的抗衰老能力。应用Draize兔眼刺激性实验评价GX-50的刺激性。结果 在组织学水平，GX-50可使高龄小鼠衰老的皮肤年轻化，增加胶原蛋白的表达。在细胞水平，GX-50可使人皮肤成纤维细胞β-半乳糖苷酶合成降低；GX-50组细胞MDA分泌量较空白对照组下降30.45%，GX-50+H₂O₂组较H2O2组下降61.46%，组间比较差异均有统计学意义（P<0.05）；GX-50组细胞胞内ROS荧光值较空白对照组下降8.03%，GX-50+H₂O₂组较H2O2组下降8.36%，组间比较差异均有统计学意义（P<0.05）。Draize兔眼刺激性评分为0。结论 GX-50可以在一定程度上保护和延缓皮肤衰老，具有作为护肤品有效添加剂的应用前景。

Anti-skin aging effect of GX-50

Objective To investigate the protective effect of GX-50 against skin aging. Methods GX-50 was applied on the back of mice aged 20 months, and the effect of GX-50 on mouse skin was observed with HE staining and immunohistochemistry. Human skin fibroblasts were primarily cultured, senescent cell model was established by H₂O₂ induction, and blank control group, DMSO group, GX-50 group, H₂O₂ group, and GX-50+H₂O₂ group (pretreatment with GX-50+H₂O₂ treatment) were divided. The anti-aging effect of GX-50 on primary human skin fibroblasts was examined by SA-β Gal staining, as well as determination of secreted malondialdehyde (MDA) concentration and intracellular reactive oxygen species (ROS) content. The irritation characteristics of GX-50 were detected by Draize eye test. Results The histological observation revealed that the GX-50 treated skin exhibited better appearance and higher collagen content. β-galactosidase in human skin fibroblasts was reduced by GX-50. Compared with blank control group, the secretion of MDA was down-regulated by 30.45% and the fluorescence value of ROS was down-regulated by 8.03% in GX-50 group (P<0.05). Compared with H₂O₂ group, the secretion of MDA was down-regulated by 61.46% and the fluorescence value of ROS was down-regulated by 8.36% in GX-50+H₂O₂ group (P<0.05). Draize eye test showed the irritation score of GX-50 was 0. Conclusion GX-50 has protective and anti-aging effect on skin, and may serve as a potential cosmetic additive against skin aging.