人类癫痫脑组织的蛋白质组学研究

目的   建立癫痫患者脑组织差异表达的蛋白质谱，进一步阐明癫痫的发病机制、寻找新的治疗靶点。方法   采用双向荧光差异凝胶电泳(2D-DIGE)和基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)对癫痫患者致痫灶脑组织差异表达的蛋白质进行分析鉴定。结果   在患者致痫灶脑组织中共鉴定出15个蛋白质点出现1.5倍以上表达差异，其中13个下调，2个上调。结论   癫痫患者脑组织中存在多个差异表达的蛋白质，可能与癫痫的发病机制相关。

Comparative proteomic study of epileptic foci inpatients with epilepsy

Objective   To construct differential express frofilings of brain tissues obtained from epilepsy patients for exploring  the pathogenic mechanism and potential therapeutic targets of epilepsy. Methods   Differentially expressed proteins in epileptic foci of six patients were identified by two-dimensional fluorescence difference gel electrophoresis(2D-DIGE) and matrix-assisted laser desorption/ionizartion time of flight mass spectrometry (MALDI-TOF-MS). Results   15 differentially  expressed proteins which showed at least 1.5 fold difference were detected in the six patients, of which 13 were down-regulated and 2 were up-regulated. Conclusion   Many differentially expressed proteins in epilepsy patients are identified, which may be involved in the pathogenesis of epilepsy.