Production and characterization of monoclonal antibodies to guinea pig lymphoid differentiation antigens |
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| Authors: | J Chiba T M Chused W M Leiserson S E Zweig E M Shevach |
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| Affiliation: | 1. Department of Chemistry, University of South Carolina, Columbia, SC 29208, U.S.A.;2. School of Medicine, University of South Carolina, Columbia, SC 29208, U.S.A. |
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| Abstract: | We have prepared 2 mouse monoclonal antibodies which react with differentiation antigens on guinea pig lymphoid cells. Monoclone 5AB2 recognizes an antigen expressed on both T and B lymphocytes and absent on macrophages. It has proven useful in the preparation of populations of antigen presenting cells which are free of T and B lymphocytes. The second monoclonal, 8BE6, is specific for peripheral T cells and 10% of thymocytes. It reacts with a 68,000 dalton molecule which is also expressed on the guinea pig B cell leukemia, EN-L2C. 8BE6 has proven to be lytic for peripheral T cells in the presence of rabbit complement and has been used to deplete T cells from heterogenous cell populations. |
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| Keywords: | quantitative immunoprecipitation reaction radioimmunoassay antigen quantitation measurement of urinary albumin BSA bovine serum albumin HSA human serum albumin PBS To whom correspondence should be addressed. |
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