针刺对戊四唑诱发癫痫大鼠海马PI3K、Akt蛋白表达的影响

目的:观察针刺对戊四唑诱发癫痫大鼠海马神经元PI3K、Akt蛋白表达的影响。方法:SD大鼠60只随机分为5组(n=12):癫痫模型组、LY294002组、针刺治疗组、针刺+LY294002组、正常对照组。各组分别于癫痫发作后4h与24h两个时间段左心灌注固定取脑组织,免疫组化法测PI3K、Akt蛋白表达。结果:针刺+LY294002组和LY294002组两个时间段均未见明显PI3K和Akt蛋白表达;癫痫模型组可见PI3K和Akt蛋白阳性表达,其中癫痫发作4h表达较24h明显(P0.01);针刺组PI3K和Akt蛋白阳性表达量明显增多,其中4h时间点蛋白阳性表达量增加更为明显(与24h时间点比较,P0.05)。结论:针刺具有明显调节PI3K和Akt蛋白表达,其作用与细胞内PI3K/Akt信号转导通路有关。

Effects of Acupuncture on Expressions of PI3K and Akt in Hippocampus of Rats with Pentylenetetrazol-Induced Epilepsy

Objective: To observe the effects of acupuncture on expressions of PI3K and Akt in hippocampus of rats with pentylenetetrazol-induced epilepsy.Methods:60 SD rats were randomly divided into 5 groups(n=12): epilepsy model group,LY294002 group,acupuncture treatment group,acupuncture treatment + LY294002 group,normal control group.4h and 24h after seizures,each group of rats were perfused with 4% paraformaldehyde,and the brain tissue were removed.PI3K and Akt protein expressions were measured with immunohistochemical method.Results: No significant expressions of PI3K and Akt in acupuncture + LY294002 group and LY294002 group during two time periods of 4h and 24h after seizures;expressions of PI3K and Akt were observed in epilepsy model group,the expression was significant at 4h compared with that at 24h(P<0.01);expression sof PI3K and Akt were significantly increased in acupuncture group,the expressions of protein were increased more significantly at 4h(compared with the that at 24h,P<0.05);Conclusion: Acupuncture has obvious regulation effects of PI3K and Akt protein expressions,the effects of regulation had relationship with intracellular PI3K / Akt signal transduction pathway.