白花蛇舌草多糖的抗氧化活性研究

目的:评价白花蛇舌草多糖的体外抗氧化能力。方法:以热水回流提取法(料水比1∶31.26,84.71℃下提取2.07h,提取2次)从白花蛇舌草(HDW)提取的多糖为材料,通过1,1-二苯基-2苦肼基自由基(DPPH)清除率、总的抗氧化活性和还原能力测定等体外抗氧化试验来评价白花蛇舌草多糖的体外抗氧化能力。结果:白花蛇舌草多糖清除DPPH自由基、总的抗氧化活性和还原能力均随多糖浓度的增加而上升;1mg/mL的多糖对DPPH自由基的清除率高达82.66%;1mg/mL多糖的总的抗氧化活性在695nm下吸光值为1.641;1mg/mL多糖的还原能力在700nm下吸光值为0.773。结论:白花蛇舌草多糖有较强的抗氧化能力,对体外自由基有较好的清除作用。

Antioxidant Activity of Polysaccharide from Hedyotis diffusa Willd.

[Objective]To evaluate the activity of polysaccharide extracted from Hedyotis diffusa Willd.in vitro.[Methods]Hedyotis diffusa Willd.Polysacchride(HDP),extracted with water at 84.71℃ for 2.07 h with Hedyotis diffusa Willd.to water ratio of 1:31.26,used as experimental material for antioxidant activity study.The antioxidant activity of polysaccharide in vitro was evaluated by the methods of scavenging DPPH radical,ORAC assay and reducing power.[Results]The results showed that the scavenging capacity of DPPH,the total antioxidant capacity and reducing power increased with the increasing concentration of HDP.1mg/mL concentration of HDP had as high as 82.66% scavenging ratio of DPPH,absorbance at 695nm of the total antioxidant capacity was 1.641 and at 700nm of reducing power was 0.773,when the concentration of HDP was 1mg/mL.[Conclusion]From the results it could be concluded that HDP had strong antioxidant capacity to have free radical scavenged in vitro.