海藻玉壶汤加减方及方中相关药物碘含量的测定

目的:研究海藻玉壶汤加减方及方中相关药物碘含量的测定及碘在复方药物提取中的变化情况。方法:在弱酸性条件下,采用重铬酸钾氧化I-为I2,氯仿定量萃取比色,测定波长为510nm。结果:总碘含量(按一个处方药物配伍比例计算)的高低依次为海藻药材海藻药材提取液海藻玉壶汤加减方及不同药物组合提取液海藻醇处理上清液海藻玉壶汤加减方中相关药材及其提取液。结论:海藻玉壶汤加减方及方中相关药物碘含量的测定结果可为制剂工艺的优选、药效研究及甲状腺疾病分型治疗提供参考依据。

Detection of Iodine in Modified Haizao Yuhu Decoction and Related Drugs within It

Objective:To study the iodine in different Chinese herbs of modified Haizao Yuhu Decoction and its quantity change under the compound extraction.Methods:Iodine was oxidated to I2 by using heavy chromium acid potassium in the weak acidic solution,then it was extracted quantitatively by chloroform and detected with UV-Vis spectrophotometry under 510nm.Results:The rank of total iodine which was calculated by one prescription proportion was Sargassum,the extraction of Sargassum,the compound extraction of modified Haizao Yuhu Decoction and different related Chinese herbs,the upper liquid of the extraction of Sargassum after adding ethanol and other related Chinese herbs and its extraction in modified Haizao Yuhu Decoction except Sargassum.Conclusion:The detection of iodine in modified Haizao Yuhu Decoction and related drugs within it could provide a reference basis for optimization of preparation process,efficacy research and the treatment of thyroid disease in different types.