| HPLC法测定红花微乳中羟基红花黄色素A的含量 |
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| 引用本文： | 季新燕,刘亚明,冯前进,赵俊云,常瑞华,冯文全,梁红娟. HPLC法测定红花微乳中羟基红花黄色素A的含量[J]. 中华中医药学刊, 2012, 0(3): 495-496 |
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| 作者姓名： | 季新燕 刘亚明 冯前进 赵俊云 常瑞华 冯文全 梁红娟 |
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| 作者单位： | 北京中医药大学;山西中医学院 |
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| 基金项目： | 国家科技攻关计划资助项目(2004BA721A46);山西省科技厅资助项目(2011091014) |
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| 摘 要： | 目的:建立HPLC法测定红花微乳中羟基红花黄色素A的含量。方法:采用色谱柱:Diamonsil C18(2)5μm,250mm×4.6mm;流动相:甲醇-0.4%的磷酸水溶液(三乙胺调至pH=3.3)为30∶70;检测波长:402nm;柱温:室温。结果:羟基红花黄色素A在0.3128～3.128μg的范围内呈良好的线性关系(r=0.9990)。结论:本测定方法快捷,简便,准确,重现性好。
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| 关 键 词： | HPLC 红花微乳 羟基红花黄色素A |
Determination of Carthamus Tinctorious-containing Microemulsion by HPLC |
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| JI Xin-yan,LIU Ya-ming,FENG Qian-jin,ZHAO Jun-yun,CHANG Rui-hua,FENG Wen-quan,LIANG Hong-juan. Determination of Carthamus Tinctorious-containing Microemulsion by HPLC[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 0(3): 495-496 |
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| Authors: | JI Xin-yan LIU Ya-ming FENG Qian-jin ZHAO Jun-yun CHANG Rui-hua FENG Wen-quan LIANG Hong-juan |
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| Affiliation: | 1.Beijing University of Chinese Medicine,Beijing 100029,China; 2.Shanxi College of Traditional Chinese Medicine,Taiyuan 030024,Shanxi,China) |
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| Abstract: | Objective:To establish a method for the determination of hydroxyl safflor yellow A(HSYA) in Carthamus tinctorious-containing microemulsion.Method:The HPLC was used with Diamonsil C18(2) column(50mm×4.6mm,5μm),detection wavelength at 402nm,mobile phase methanol-water(pH3.3) 30∶ 70,flow rate:0.8mL/min.Results:HSYA has good linear relationship between 0.3128~3.128μg,r=0.9990,the recovery of HSYA was 99.93% and RSD was 1.86%(n=6).Conclusion:The method is simple,sensitive and reliable to carry out to control the assay of Carthamus tinctorious-containing microemulsion transdermal delivery system. |
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| Keywords: | HPLC Carthamus tinctorious-containing microemulsion hydroxyl safflor yellow A |
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