乳腺癌层粘连蛋白的免疫组织化学研究

本研究应用层粘连蛋白（ＬＮ）抗体，对２７例乳腺癌、５例乳腺良性病变进行了免疫组织化学研究。结果发现乳腺良性病变时，ＬＮ以均匀完整的线型阳性反应显示在乳腺导管及小叶基膜处。乳腺癌时，ＬＮ的染色表现为癌细胞胞浆内弥漫状阳性反应。２７例乳腺癌中，癌细胞ＬＮ染色强度不同，伴有转移的乳腺癌ＬＮ染色多数较强，强度在＋＋～的为７／１２（５８．３％）；不伴有转移的病例，ＬＮ染色多数较浅或为阴性，强度在＋＋～＋＋＋的病例为３／１５（２０％）。说明癌细胞内源性ＬＮ染色强弱与肿瘤转移密切相关，内源性ＬＮ的合成可能改变肿瘤细胞的浸润活性。

Immunohistoch emical study on breast cancer for Laminin

In this study,twenty- seven in infiltrating ductal carcinomas of breast and five be-nign human breasts were immunohistochemically examined for the presence and intensity of LN.We observed the LN staining intensity in breast carcinomas with or without metastasis.The re-sults showed that in the benign human breast, a positive LN staining was present in basementmembrane of the epithclia,showing a linear continuous pattern.In breast with carcinom a,a pos-itive LN staining was found in the cytoplasm. Intracellular LN positive staining intensity wasvarious in these 27 cases, In most cases with metastasis,LN positive staining was strong, withthe staining intensity of++～+++found in7/12 (58.3%).In most cases without metastasis,L Nstaining was negative or weakly positive,with the staining intensity of++～+++found in only 3/15(20%).It suggested that there was a relationship between the endogenous LN in the cancercells and metastasis.Increased synthesis of the endogenous LN may change the infiltrating activity of cancer cells,